Characterization of hemodialysis membranes by inverse size exclusion chromatography

A.P. Broek, Arnold P. Broek, Herman A. Teunis, Hermannus A. Teunis, D. Bargeman, Derk Bargeman, Erik D. Sprengers, H. Strathmann, C.A. Smolders, C.A. Smolders

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Inverse size exclusion chromatography (i-SEC) was used to characterize three different cellulosic hollow fiber hemodialysis membranes, i.e. low-flux cuprophan and hemophan and high-flux RC-HP400A. With the i-SEC technique the pore size distribution and porosity of a membrane can be determined and adsorption phenomena can be studied. The membranes showed clear differences in pore size and porosity, the high-flux RC-HP400A membrane has a larger pore size as well as a higher porosity. For all the membranes it was found that the elution curves were best described by a homoporous pore volume distribution. It appeared that the bound or non-freezing water in the membranes was at least partly accessible to solutes. The test molecules creatinine and vitamin B 12 both adsorbed to the cellulosic membranes. The adsorption behavior of creatinine was strongly dependent on the NaCl concentration present. The observations could be explained by assuming that cuprophan and RC-HP400A are negatively charged whereas hemophan is positively charged due to the modification with N,N-diethylaminoethyl ether. The net charge of the hemophan is smaller.
Original languageUndefined
Pages (from-to)217-228
Number of pages12
JournalJournal of membrane science
Issue number99
Publication statusPublished - 1995


  • METIS-106922
  • IR-12741

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