Characterization of hemodialysis membranes by inverse size exclusion chromatography

Arnold P. Broek, Herman A. Teunis, Derk Bargeman, Erik D. Sprengers, Heiner Strathmann, Cees A. Smolders

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)
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Inverse size exclusion chromatography (i-SEC) was used to characterize three different cellulosic hollow fiber hemodialysis membranes, i.e. low-flux cuprophan and hemophan and high-flux RC-HP400A. With the i-SEC technique the pore size distribution and porosity of a membrane can be determined and adsorption phenomena can be studied. The membranes showed clear differences in pore size and porosity, the high-flux RC-HP400A membrane has a larger pore size as well as a higher porosity. For all the membranes it was found that the elution curves were best described by a homoporous pore volume distribution. It appeared that the bound or non-freezing water in the membranes was at least partly accessible to solutes. The test molecules creatinine and vitamin B 12 both adsorbed to the cellulosic membranes. The adsorption behavior of creatinine was strongly dependent on the NaCl concentration present. The observations could be explained by assuming that cuprophan and RC-HP400A are negatively charged whereas hemophan is positively charged due to the modification with N,N-diethylaminoethyl ether. The net charge of the hemophan is smaller.
Original languageEnglish
Pages (from-to)217-228
Number of pages12
JournalJournal of membrane science
Issue number3
Publication statusPublished - 1995


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