TY - JOUR
T1 - Characterization of human embryonic stem cell lines by the International Stem Cell Initiative
AU - Adewumi, Oluseun
AU - Aflatoonian, Behrouz
AU - Ahrlund-Richter, Lars
AU - Amit, Michal
AU - Andrews, Peter W.
AU - Beighton, Gemma
AU - Bello, Paul A.
AU - Benvenisty, Nissim
AU - Berry, Lorraine S.
AU - Bevan, Simon
AU - Blum, Barak
AU - Brooking, Justin
AU - Chen, Kevin G.
AU - Choo, Andre B.H.
AU - Churchill, Gary A.
AU - Corbel, Marie
AU - Damjanov, Ivan
AU - Draper, Jon S.
AU - Dvorak, Petr
AU - Emanuelsson, Katarina
AU - Fleck, Roland A.
AU - Ford, Angela
AU - Gertow, Karin
AU - Gertsenstein, Marina
AU - Gokhale, Paul J.
AU - Hamilton, Rebecca S.
AU - Hampl, Ales
AU - Healy, Lyn E.
AU - Hovatta, Outi
AU - Hyllner, Johan
AU - Imreh, Marta P.
AU - Itskovitz-Eldor, Joseph
AU - Jackson, Jamie
AU - Johnson, Jacqueline L.
AU - Jones, Mark
AU - Kee, Kehkooi
AU - King, Benjamin L.
AU - Knowles, Barbara B.
AU - Lako, Majlinda
AU - Lebrin, Franck
AU - Mallon, Barbara S.
AU - Manning, Daisy
AU - Mayshar, Yoav
AU - McKay, Ronald D.G.
AU - Michalska, Anna E.
AU - Mikkola, Milla
AU - Mileikovsky, Masha
AU - Minger, Stephen L.
AU - Mummery, Christine L.
AU - Passier, Robert
AU - The International Stem Cell Initiative
PY - 2007/1/1
Y1 - 2007/1/1
N2 - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
AB - The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.
UR - http://www.scopus.com/inward/record.url?scp=34447295350&partnerID=8YFLogxK
U2 - 10.1038/nbt1318
DO - 10.1038/nbt1318
M3 - Article
C2 - 17572666
AN - SCOPUS:34447295350
SN - 1087-0156
VL - 25
SP - 803
EP - 816
JO - Nature biotechnology
JF - Nature biotechnology
IS - 7
ER -