TY - JOUR
T1 - Characterization of magnetosomes after exposure to the effect of the Sonication and ultracentrifugation
AU - Molčan, M.
AU - Hashim, A.
AU - Kováč, J.
AU - Rajňák, M.
AU - Kopčanský, P.
AU - Makowski, M.
AU - Gojzewski, H.
AU - Molokác, M.
AU - Hvizdák, L.
AU - Timko, M.
N1 - Conference code: 15
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Magnetosomes are intracellular organelles of widespread aquatic microorganisms called Magnetotactic bacteria. At present they are under investigation especially in biomedical applications. This ability depends on the presence of intracellular magnetosomes which are composed of two parts: first, nanometer-sized magnetite (Fe3O4) or greigite (Fe 3S4) crystals (magnetosome crystal), depending on the bacterial species; and second, the bilayer membrane surrounding the crystal (magnetosome membrane). The magnetosomes were prepared by biomineralization process of magnetotactic bacteria Magnetospirillum Magnetotacticum sp. AMB-1. The isolated magnetosome chains (sample M) were centrifugated at speed of 100000 rpm for 4 hours (sample UM) and sonicated at power of 120 W for 3 hours (sample SM), respectively. The prepared suspensions were investigated with respect to morphological, structural and magnetic properties. The results from scanning electron microscopy showed that isolated chains of magnetosomes were partially broken to smaller ones after ultracentrifugation. On the other hand the application of the sonication process caused the formation of individual magnetosomes (unordered in chain). These results were confirmed by coercivity and magnetization saturation measurements.
AB - Magnetosomes are intracellular organelles of widespread aquatic microorganisms called Magnetotactic bacteria. At present they are under investigation especially in biomedical applications. This ability depends on the presence of intracellular magnetosomes which are composed of two parts: first, nanometer-sized magnetite (Fe3O4) or greigite (Fe 3S4) crystals (magnetosome crystal), depending on the bacterial species; and second, the bilayer membrane surrounding the crystal (magnetosome membrane). The magnetosomes were prepared by biomineralization process of magnetotactic bacteria Magnetospirillum Magnetotacticum sp. AMB-1. The isolated magnetosome chains (sample M) were centrifugated at speed of 100000 rpm for 4 hours (sample UM) and sonicated at power of 120 W for 3 hours (sample SM), respectively. The prepared suspensions were investigated with respect to morphological, structural and magnetic properties. The results from scanning electron microscopy showed that isolated chains of magnetosomes were partially broken to smaller ones after ultracentrifugation. On the other hand the application of the sonication process caused the formation of individual magnetosomes (unordered in chain). These results were confirmed by coercivity and magnetization saturation measurements.
UR - http://www.scopus.com/inward/record.url?scp=84902152267&partnerID=8YFLogxK
U2 - 10.12693/APhysPolA.126.198
DO - 10.12693/APhysPolA.126.198
M3 - Article
AN - SCOPUS:84902152267
SN - 0587-4246
VL - 126
SP - 198
EP - 199
JO - Acta physica polonica A
JF - Acta physica polonica A
IS - 1
T2 - 15th Czech and Slovak Conference on Magnetism 2013
Y2 - 17 June 2013 through 21 June 2013
ER -