Characterization of microbubble-loaded stem cells for targeted cell therapy

Tom J.A. Kokhuis, Ilya Skachkov, Benno Naaijkens, Lynda J.M. Juffermans, Otto Kamp, Antonius F.W. Van Der Steen, Michel Versluis, Nico De Jong

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is that only a small fraction of the transplanted cells engraft in the infarcted area of the heart. In this study we investigated the use of stem cells conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells, to achieve increased stem cell delivery. The dynamics of the constructs in response to an ultrasound wave was characterized using high speed optical imaging. A modified Rayleigh-Plesset equation was developed to corroborate our experimental findings. We show that acoustic radiation force can be used to propel StemBells to the wall of a 200 μm vessel in a chicken embryo. This technique therefore facilitates the possibility to localize stem cell delivery to increase the engraftment and improve the clinical outcome of stem cell therapy.

Original languageEnglish
Title of host publication2013 IEEE International Ultrasonics Symposium, IUS 2013
Pages1146-1149
Number of pages4
DOIs
Publication statusPublished - 1 Dec 2013

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Fingerprint

stem cells
therapy
cells
delivery
Rayleigh equations
myocardial infarction
chickens
embryos
sound waves
vessels
high speed

Keywords

  • Acoustic radiation force
  • Bjerknes force
  • Cardiac repair
  • Stem cell therapy
  • Targeted microbubble

Cite this

Kokhuis, T. J. A., Skachkov, I., Naaijkens, B., Juffermans, L. J. M., Kamp, O., Van Der Steen, A. F. W., ... De Jong, N. (2013). Characterization of microbubble-loaded stem cells for targeted cell therapy. In 2013 IEEE International Ultrasonics Symposium, IUS 2013 (pp. 1146-1149). [6724853] (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2013.0293
Kokhuis, Tom J.A. ; Skachkov, Ilya ; Naaijkens, Benno ; Juffermans, Lynda J.M. ; Kamp, Otto ; Van Der Steen, Antonius F.W. ; Versluis, Michel ; De Jong, Nico. / Characterization of microbubble-loaded stem cells for targeted cell therapy. 2013 IEEE International Ultrasonics Symposium, IUS 2013. 2013. pp. 1146-1149 (IEEE International Ultrasonics Symposium, IUS).
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abstract = "The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is that only a small fraction of the transplanted cells engraft in the infarcted area of the heart. In this study we investigated the use of stem cells conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells, to achieve increased stem cell delivery. The dynamics of the constructs in response to an ultrasound wave was characterized using high speed optical imaging. A modified Rayleigh-Plesset equation was developed to corroborate our experimental findings. We show that acoustic radiation force can be used to propel StemBells to the wall of a 200 μm vessel in a chicken embryo. This technique therefore facilitates the possibility to localize stem cell delivery to increase the engraftment and improve the clinical outcome of stem cell therapy.",
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Kokhuis, TJA, Skachkov, I, Naaijkens, B, Juffermans, LJM, Kamp, O, Van Der Steen, AFW, Versluis, M & De Jong, N 2013, Characterization of microbubble-loaded stem cells for targeted cell therapy. in 2013 IEEE International Ultrasonics Symposium, IUS 2013., 6724853, IEEE International Ultrasonics Symposium, IUS, pp. 1146-1149. https://doi.org/10.1109/ULTSYM.2013.0293

Characterization of microbubble-loaded stem cells for targeted cell therapy. / Kokhuis, Tom J.A.; Skachkov, Ilya; Naaijkens, Benno; Juffermans, Lynda J.M.; Kamp, Otto; Van Der Steen, Antonius F.W.; Versluis, Michel; De Jong, Nico.

2013 IEEE International Ultrasonics Symposium, IUS 2013. 2013. p. 1146-1149 6724853 (IEEE International Ultrasonics Symposium, IUS).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Kokhuis TJA, Skachkov I, Naaijkens B, Juffermans LJM, Kamp O, Van Der Steen AFW et al. Characterization of microbubble-loaded stem cells for targeted cell therapy. In 2013 IEEE International Ultrasonics Symposium, IUS 2013. 2013. p. 1146-1149. 6724853. (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2013.0293