TY - GEN
T1 - Characterization of microbubble-loaded stem cells for targeted cell therapy
AU - Kokhuis, Tom J.A.
AU - Skachkov, Ilya
AU - Naaijkens, Benno
AU - Juffermans, Lynda J.M.
AU - Kamp, Otto
AU - Van Der Steen, Antonius F.W.
AU - Versluis, Michel
AU - De Jong, Nico
PY - 2013/12/1
Y1 - 2013/12/1
N2 - The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is that only a small fraction of the transplanted cells engraft in the infarcted area of the heart. In this study we investigated the use of stem cells conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells, to achieve increased stem cell delivery. The dynamics of the constructs in response to an ultrasound wave was characterized using high speed optical imaging. A modified Rayleigh-Plesset equation was developed to corroborate our experimental findings. We show that acoustic radiation force can be used to propel StemBells to the wall of a 200 μm vessel in a chicken embryo. This technique therefore facilitates the possibility to localize stem cell delivery to increase the engraftment and improve the clinical outcome of stem cell therapy.
AB - The use of stem cells for the repair of damaged cardiac tissue after a myocardial infarction holds great promise. However, a common finding in experimental studies is that only a small fraction of the transplanted cells engraft in the infarcted area of the heart. In this study we investigated the use of stem cells conjugated with targeted microbubbles, creating echogenic complexes dubbed StemBells, to achieve increased stem cell delivery. The dynamics of the constructs in response to an ultrasound wave was characterized using high speed optical imaging. A modified Rayleigh-Plesset equation was developed to corroborate our experimental findings. We show that acoustic radiation force can be used to propel StemBells to the wall of a 200 μm vessel in a chicken embryo. This technique therefore facilitates the possibility to localize stem cell delivery to increase the engraftment and improve the clinical outcome of stem cell therapy.
KW - Acoustic radiation force
KW - Bjerknes force
KW - Cardiac repair
KW - Stem cell therapy
KW - Targeted microbubble
UR - http://www.scopus.com/inward/record.url?scp=84894299946&partnerID=8YFLogxK
U2 - 10.1109/ULTSYM.2013.0293
DO - 10.1109/ULTSYM.2013.0293
M3 - Conference contribution
AN - SCOPUS:84894299946
SN - 9781467356862
T3 - IEEE International Ultrasonics Symposium, IUS
SP - 1146
EP - 1149
BT - 2013 IEEE International Ultrasonics Symposium, IUS 2013
T2 - 2013 IEEE International Ultrasonics Symposium, IUS 2013
Y2 - 21 July 2013 through 25 July 2013
ER -