Characterization of necrotic and viable spermatozoa in microfluidic impedance cytrometry

Stella A. Kruit*, Douwe Sietse de Bruijn, Wouter Olthuis, Loes Segerink

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

The viability and acrosomal status of spermatozoa are important factors for male fertility prediction. Dye exclusion is a common method to determine these factors. In our search for label-free methods, we used microfluidic impedance cytometry to measure differences between necrotic and viable spermatozoa. At low frequency, a significant (p < 0.05) smaller mean impedance magnitude for necrotic cells was measured compared to viable cells. Further investigation is needed to verify the impact of acrosomal status on the impedance. However, these results indicate that microfluidic impedance cytometry has the potential to distinguish between viable and necrotic/acrosome reacted spermatozoa.
Original languageEnglish
Title of host publication25th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Subtitle of host publication10 - 14 October 2021, Palm Springs, CA, USA
Pages549-550
Publication statusPublished - 11 Oct 2021
Event25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States
Duration: 10 Oct 202114 Oct 2021
Conference number: 25
https://microtas2021.org/

Conference

Conference25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021
Abbreviated titleMicroTAS 2021
Country/TerritoryUnited States
CityPalm Springs
Period10/10/2114/10/21
Internet address

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