Abstract
The viability and acrosomal status of spermatozoa are important factors for male fertility prediction. Dye exclusion is a common method to determine these factors. In our search for label-free methods, we used microfluidic impedance cytometry to measure differences between necrotic and viable spermatozoa. At low frequency, a significant (p < 0.05) smaller mean impedance magnitude for necrotic cells was measured compared to viable cells. Further investigation is needed to verify the impact of acrosomal status on the impedance. However, these results indicate that microfluidic impedance cytometry has the potential to distinguish between viable and necrotic/acrosome reacted spermatozoa.
Original language | English |
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Title of host publication | MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
Publisher | The Chemical and Biological Microsystems Society |
Pages | 549-550 |
Number of pages | 2 |
ISBN (Electronic) | 9781733419031 |
Publication status | Published - 2021 |
Event | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 - Palm Springs, United States Duration: 10 Oct 2021 → 14 Oct 2021 Conference number: 25 https://microtas2021.org/ |
Conference
Conference | 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, µTAS 2021 |
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Abbreviated title | MicroTAS 2021 |
Country/Territory | United States |
City | Palm Springs |
Period | 10/10/21 → 14/10/21 |
Internet address |
Keywords
- Artificial Insemination
- Electrical Impedance Cytometry
- Microfluidics
- Spermatozoa