TY - JOUR
T1 - Characterization of polyurethane scaffold surface functionalization with diamines and heparin
AU - de Mulder, Eric L.W.
AU - Hannink, Gerjon
AU - Koens, Martin J.W.
AU - Löwik, Dennis W.P.M.
AU - Verdonschot, Nicolaas Jacobus Joseph
AU - Buma, Pieter
PY - 2013
Y1 - 2013
N2 - Polyurethane scaffolds (PUs) have a good biocompatibility but lack cell recognition sites. In this study, we functionalized the surface of a PU, P(D/L)LA and PCL (50:50) containing urethane segments, with heparin. The first step in this functionalization, aminolysis, lead to free amine groups on the surface of the PU. Free amine content was determined to be 6.4 nmol/mL/mg scaffold, a significant increase of 230%. Subsequently, heparin was crosslinked. Immunohistochemistry demonstrated the presence of heparin homogeneous throughout the 3D porous scaffold. Young's modulus decreased significantly till 50% of the native stiffness after aminolysis and did not change after heparin crosslinking. Contact angle on PU films significantly decreased from 82.7° to 64.3° after heparin crosslinking, indicating a more hydrophilic surface. This functionalization beholds great potential for tissue engineering purposes. When used in a load-bearing environment, caution is necessary due to reduction in mechanical stiffness
AB - Polyurethane scaffolds (PUs) have a good biocompatibility but lack cell recognition sites. In this study, we functionalized the surface of a PU, P(D/L)LA and PCL (50:50) containing urethane segments, with heparin. The first step in this functionalization, aminolysis, lead to free amine groups on the surface of the PU. Free amine content was determined to be 6.4 nmol/mL/mg scaffold, a significant increase of 230%. Subsequently, heparin was crosslinked. Immunohistochemistry demonstrated the presence of heparin homogeneous throughout the 3D porous scaffold. Young's modulus decreased significantly till 50% of the native stiffness after aminolysis and did not change after heparin crosslinking. Contact angle on PU films significantly decreased from 82.7° to 64.3° after heparin crosslinking, indicating a more hydrophilic surface. This functionalization beholds great potential for tissue engineering purposes. When used in a load-bearing environment, caution is necessary due to reduction in mechanical stiffness
KW - METIS-301808
KW - IR-90981
U2 - 10.1002/jbm.a.34389
DO - 10.1002/jbm.a.34389
M3 - Article
SN - 1549-3296
VL - 101A
SP - 919
EP - 922
JO - Journal of biomedical materials research. Part A
JF - Journal of biomedical materials research. Part A
IS - 4
ER -