Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging

Ying Luan, Erik Gelderblom, Guillaume Lajoinie, Ilya Skachkov, Heleen Dewitte, Bart Geers, Ine Lentacker, Ton Van Der Steen, Michel Versluis, Nico De Jong

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Ultrasound contrast agents (UCAs) microbubbles (MBs) can be preloaded with a therapeutic agents to achieve high efficiency for US-triggered drug delivery. Here we use fluorescence labeling as a substitute for theraputic drug, and use ultra high-speed fluorescence imaging for time-resolved observation of the drug release. Two configurations of drug delivery vehicles were investigated-I) lipid-shelled (unloaded) MBs and II) liposome-loaded (loaded) MBs. Different release phenomena were observed. The dynamics of release was found to be strongly dependent on ultrasonic parameters and on the MB's shell properties. MBs shrinkage following US exposure was analyzed and it indicated close correlation with the fluorescence release. This study provides valuable insights into the drug release mechanisms for ultrasound-controlled drug delivery.

Original languageEnglish
Title of host publication2012 IEEE International Ultrasonics Symposium, IUS 2012
DOIs
Publication statusPublished - 1 Dec 2012
EventIEEE International Ultrasonics Symposium, IUS 2012 - Dresden, Germany
Duration: 7 Oct 201210 Oct 2012

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Conference

ConferenceIEEE International Ultrasonics Symposium, IUS 2012
Abbreviated titleIUS 2012
CountryGermany
CityDresden
Period7/10/1210/10/12

Fingerprint

drugs
high speed
fluorescence
delivery
shrinkage
marking
lipids
vehicles
ultrasonics
substitutes
configurations

Keywords

  • controlled release
  • drug delivery
  • fluorescence labeling
  • high-speed fluorescence imaging
  • release dynamics

Cite this

Luan, Y., Gelderblom, E., Lajoinie, G., Skachkov, I., Dewitte, H., Geers, B., ... De Jong, N. (2012). Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging. In 2012 IEEE International Ultrasonics Symposium, IUS 2012 [6562061] (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2012.0510
Luan, Ying ; Gelderblom, Erik ; Lajoinie, Guillaume ; Skachkov, Ilya ; Dewitte, Heleen ; Geers, Bart ; Lentacker, Ine ; Van Der Steen, Ton ; Versluis, Michel ; De Jong, Nico. / Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging. 2012 IEEE International Ultrasonics Symposium, IUS 2012. 2012. (IEEE International Ultrasonics Symposium, IUS).
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title = "Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging",
abstract = "Ultrasound contrast agents (UCAs) microbubbles (MBs) can be preloaded with a therapeutic agents to achieve high efficiency for US-triggered drug delivery. Here we use fluorescence labeling as a substitute for theraputic drug, and use ultra high-speed fluorescence imaging for time-resolved observation of the drug release. Two configurations of drug delivery vehicles were investigated-I) lipid-shelled (unloaded) MBs and II) liposome-loaded (loaded) MBs. Different release phenomena were observed. The dynamics of release was found to be strongly dependent on ultrasonic parameters and on the MB's shell properties. MBs shrinkage following US exposure was analyzed and it indicated close correlation with the fluorescence release. This study provides valuable insights into the drug release mechanisms for ultrasound-controlled drug delivery.",
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author = "Ying Luan and Erik Gelderblom and Guillaume Lajoinie and Ilya Skachkov and Heleen Dewitte and Bart Geers and Ine Lentacker and {Van Der Steen}, Ton and Michel Versluis and {De Jong}, Nico",
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Luan, Y, Gelderblom, E, Lajoinie, G, Skachkov, I, Dewitte, H, Geers, B, Lentacker, I, Van Der Steen, T, Versluis, M & De Jong, N 2012, Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging. in 2012 IEEE International Ultrasonics Symposium, IUS 2012., 6562061, IEEE International Ultrasonics Symposium, IUS, IEEE International Ultrasonics Symposium, IUS 2012, Dresden, Germany, 7/10/12. https://doi.org/10.1109/ULTSYM.2012.0510

Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging. / Luan, Ying; Gelderblom, Erik; Lajoinie, Guillaume; Skachkov, Ilya; Dewitte, Heleen; Geers, Bart; Lentacker, Ine; Van Der Steen, Ton; Versluis, Michel; De Jong, Nico.

2012 IEEE International Ultrasonics Symposium, IUS 2012. 2012. 6562061 (IEEE International Ultrasonics Symposium, IUS).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Luan Y, Gelderblom E, Lajoinie G, Skachkov I, Dewitte H, Geers B et al. Characterizing ultrasound-controlled drug release by high-speed fluorescence imaging. In 2012 IEEE International Ultrasonics Symposium, IUS 2012. 2012. 6562061. (IEEE International Ultrasonics Symposium, IUS). https://doi.org/10.1109/ULTSYM.2012.0510