Charged porous membrane structures for separation of biomolecules

Karina Katarzyna Kopec

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

43 Downloads (Pure)

Abstract

Thesis presents various membrane techniques for biomolecules separation. New charged membranes and new methods to introduce charge into the membranes are demonstrated. All chapters present characterization of the hollow fiber membranes produced via the immersion precipitation dry-wet spinning. Apart from the spinning technique, the phase separation in-situ and polymer foaming are explored, too. A model protein or a model protein mixture is investigated in each chapter to give a proof of concept of the new method. Bovine serum albumin is used, as this protein is known of its stability, lack of effect in many biochemical reactions and low cost. First, a new in-line method of introduction charge onto the bore surface of the hollow fiber membrane in a one-step spinning process is described. A negatively charged polyelectrolyte sulphonated poly(ether ether ketone) (SPEEK) is dissolved in the bore liquid and during the membrane formation deposited on the bore surface. Secondly, a crosslinking agent for the membrane forming polymer, the polyethyleneimine (PEI) is dissolved in the bore liquid. This is a new method of simultaneous membrane formation and chemical modification in a one-step spinning process. It is shown that two types of membranes: either a completely crosslinked and porous fiber (for ultrafiltration), or a porous fiber with crosslinked dense inner layer (for gas separation), can be produced via the same spinning procedure, only by changing the composition of the bore liquid and/or shell liquid and dope solution. Third method explores application of mixed matrix membranes for adsorption of biomolecules. These membranes are produced by dispersion of negatively charged particles in the matrix polymer. The novelty of this work is that the membranes are crosslinked and for the first time applied for the adsorption of biomolecules (lysozyme and cholesterol) from organic solvents, rather than from aqueous solutions. The last part introduces membrane technology in a completely new field, capillary elelectrochromatography (CEC) – a hybrid analytical separation technique. Here, membrane fabrication methods described previously, together with new methods, are applied for preparation of novel stationary phases for CEC columns, which are tested in a laboratorydesigned and laboratory-built CEC unit.
Original languageEnglish
Awarding Institution
  • University of Twente
Supervisors/Advisors
  • Wessling, Matthias , Supervisor
  • Stamatialis, D., Co-Supervisor
Award date28 Jan 2011
Place of PublicationEnschede
Publisher
Print ISBNs978-90-365-3146-7
DOIs
Publication statusPublished - 28 Jan 2011

Fingerprint

Membrane structures
Biomolecules
Membranes
Fibers
Polymers
Adsorption
Membrane technology
Polyetherimides
Polyether ether ketones
Chemical modification
Liquids
Charged particles
Muramidase
Bovine Serum Albumin
Polymer matrix
Crosslinking
Proteins
Gases
Cholesterol

Keywords

  • IR-75805
  • METIS-279612

Cite this

Kopec, Karina Katarzyna. / Charged porous membrane structures for separation of biomolecules. Enschede : University of Twente, 2011. 155 p.
@phdthesis{8156fcc5debb48f584cc28d0476a1ad3,
title = "Charged porous membrane structures for separation of biomolecules",
abstract = "Thesis presents various membrane techniques for biomolecules separation. New charged membranes and new methods to introduce charge into the membranes are demonstrated. All chapters present characterization of the hollow fiber membranes produced via the immersion precipitation dry-wet spinning. Apart from the spinning technique, the phase separation in-situ and polymer foaming are explored, too. A model protein or a model protein mixture is investigated in each chapter to give a proof of concept of the new method. Bovine serum albumin is used, as this protein is known of its stability, lack of effect in many biochemical reactions and low cost. First, a new in-line method of introduction charge onto the bore surface of the hollow fiber membrane in a one-step spinning process is described. A negatively charged polyelectrolyte sulphonated poly(ether ether ketone) (SPEEK) is dissolved in the bore liquid and during the membrane formation deposited on the bore surface. Secondly, a crosslinking agent for the membrane forming polymer, the polyethyleneimine (PEI) is dissolved in the bore liquid. This is a new method of simultaneous membrane formation and chemical modification in a one-step spinning process. It is shown that two types of membranes: either a completely crosslinked and porous fiber (for ultrafiltration), or a porous fiber with crosslinked dense inner layer (for gas separation), can be produced via the same spinning procedure, only by changing the composition of the bore liquid and/or shell liquid and dope solution. Third method explores application of mixed matrix membranes for adsorption of biomolecules. These membranes are produced by dispersion of negatively charged particles in the matrix polymer. The novelty of this work is that the membranes are crosslinked and for the first time applied for the adsorption of biomolecules (lysozyme and cholesterol) from organic solvents, rather than from aqueous solutions. The last part introduces membrane technology in a completely new field, capillary elelectrochromatography (CEC) – a hybrid analytical separation technique. Here, membrane fabrication methods described previously, together with new methods, are applied for preparation of novel stationary phases for CEC columns, which are tested in a laboratorydesigned and laboratory-built CEC unit.",
keywords = "IR-75805, METIS-279612",
author = "Kopec, {Karina Katarzyna}",
year = "2011",
month = "1",
day = "28",
doi = "10.3990/1.9789036531467",
language = "English",
isbn = "978-90-365-3146-7",
publisher = "University of Twente",
address = "Netherlands",
school = "University of Twente",

}

Charged porous membrane structures for separation of biomolecules. / Kopec, Karina Katarzyna.

Enschede : University of Twente, 2011. 155 p.

Research output: ThesisPhD Thesis - Research UT, graduation UTAcademic

TY - THES

T1 - Charged porous membrane structures for separation of biomolecules

AU - Kopec, Karina Katarzyna

PY - 2011/1/28

Y1 - 2011/1/28

N2 - Thesis presents various membrane techniques for biomolecules separation. New charged membranes and new methods to introduce charge into the membranes are demonstrated. All chapters present characterization of the hollow fiber membranes produced via the immersion precipitation dry-wet spinning. Apart from the spinning technique, the phase separation in-situ and polymer foaming are explored, too. A model protein or a model protein mixture is investigated in each chapter to give a proof of concept of the new method. Bovine serum albumin is used, as this protein is known of its stability, lack of effect in many biochemical reactions and low cost. First, a new in-line method of introduction charge onto the bore surface of the hollow fiber membrane in a one-step spinning process is described. A negatively charged polyelectrolyte sulphonated poly(ether ether ketone) (SPEEK) is dissolved in the bore liquid and during the membrane formation deposited on the bore surface. Secondly, a crosslinking agent for the membrane forming polymer, the polyethyleneimine (PEI) is dissolved in the bore liquid. This is a new method of simultaneous membrane formation and chemical modification in a one-step spinning process. It is shown that two types of membranes: either a completely crosslinked and porous fiber (for ultrafiltration), or a porous fiber with crosslinked dense inner layer (for gas separation), can be produced via the same spinning procedure, only by changing the composition of the bore liquid and/or shell liquid and dope solution. Third method explores application of mixed matrix membranes for adsorption of biomolecules. These membranes are produced by dispersion of negatively charged particles in the matrix polymer. The novelty of this work is that the membranes are crosslinked and for the first time applied for the adsorption of biomolecules (lysozyme and cholesterol) from organic solvents, rather than from aqueous solutions. The last part introduces membrane technology in a completely new field, capillary elelectrochromatography (CEC) – a hybrid analytical separation technique. Here, membrane fabrication methods described previously, together with new methods, are applied for preparation of novel stationary phases for CEC columns, which are tested in a laboratorydesigned and laboratory-built CEC unit.

AB - Thesis presents various membrane techniques for biomolecules separation. New charged membranes and new methods to introduce charge into the membranes are demonstrated. All chapters present characterization of the hollow fiber membranes produced via the immersion precipitation dry-wet spinning. Apart from the spinning technique, the phase separation in-situ and polymer foaming are explored, too. A model protein or a model protein mixture is investigated in each chapter to give a proof of concept of the new method. Bovine serum albumin is used, as this protein is known of its stability, lack of effect in many biochemical reactions and low cost. First, a new in-line method of introduction charge onto the bore surface of the hollow fiber membrane in a one-step spinning process is described. A negatively charged polyelectrolyte sulphonated poly(ether ether ketone) (SPEEK) is dissolved in the bore liquid and during the membrane formation deposited on the bore surface. Secondly, a crosslinking agent for the membrane forming polymer, the polyethyleneimine (PEI) is dissolved in the bore liquid. This is a new method of simultaneous membrane formation and chemical modification in a one-step spinning process. It is shown that two types of membranes: either a completely crosslinked and porous fiber (for ultrafiltration), or a porous fiber with crosslinked dense inner layer (for gas separation), can be produced via the same spinning procedure, only by changing the composition of the bore liquid and/or shell liquid and dope solution. Third method explores application of mixed matrix membranes for adsorption of biomolecules. These membranes are produced by dispersion of negatively charged particles in the matrix polymer. The novelty of this work is that the membranes are crosslinked and for the first time applied for the adsorption of biomolecules (lysozyme and cholesterol) from organic solvents, rather than from aqueous solutions. The last part introduces membrane technology in a completely new field, capillary elelectrochromatography (CEC) – a hybrid analytical separation technique. Here, membrane fabrication methods described previously, together with new methods, are applied for preparation of novel stationary phases for CEC columns, which are tested in a laboratorydesigned and laboratory-built CEC unit.

KW - IR-75805

KW - METIS-279612

U2 - 10.3990/1.9789036531467

DO - 10.3990/1.9789036531467

M3 - PhD Thesis - Research UT, graduation UT

SN - 978-90-365-3146-7

PB - University of Twente

CY - Enschede

ER -