The internal walls of microfabricated fluidic channels were functionalized with a selfassembled monolayer of Rhodamine B lactam. This molecule has the capability to interconvert between its open fluorescent amide form and the closed non-fluorescent lactam form upon changes of the pH conditions. The interconversion (switch) between the two reversible forms is achieved by addition of an acid or a base and is consistent with a reaction mechanism of the first order. This paper describes the online observation of such fluorescent switch covalently anchored to the channel and proposes this method as a possible sensor for the monitoring of pH changes in microreactors.
|Title of host publication||Proceedings of the 17th European Conference on Solid-State Transducers (EUROSENSORS)|
|Place of Publication||Braga, Portugal|
|Publisher||Barbarosa & Xavier|
|Number of pages||2|
|Publication status||Published - 2003|
|Event||Eurosensors XVII, European Conference on Solid-State Transducers - Guimaraes, Portugal|
Duration: 21 Sep 2003 → 24 Sep 2003
|Conference||Eurosensors XVII, European Conference on Solid-State Transducers|
|Period||21/09/03 → 24/09/03|
Mela, P., Onclin, S., Goedbloed, M. H., Levi, S., van Hulst, N. F., & van den Berg, A. (2003). Chemically driven switches for online detection of pH changes in microfluidic devices. In Proceedings of the 17th European Conference on Solid-State Transducers (EUROSENSORS) (pp. 21-22). Braga, Portugal: Barbarosa & Xavier.