Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering

Sepehr Talebian; Mehdi Mehrali; Saktiswaren Mohan; Hanumantha Rao Balaji Raghavendran; Mohammad Mehrali; Hossein Mohammad Khanlou; Tunku Kamarul; Amalina Muhammad Afifi; Azlina Amir Abass

doi:10.1039/c4ra06761d

Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering

Sepehr Talebian^*, Mehdi Mehrali, Saktiswaren Mohan, Hanumantha Rao Balaji Raghavendran, Mohammad Mehrali, Hossein Mohammad Khanlou, Tunku Kamarul, Amalina Muhammad Afifi, Azlina Amir Abass

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

A novel hybrid nanofibrous scaffold prepared with chitosan [containing 1.2 wt% polyethylene oxide (PEO)] and bioactive glass (BG) was fabricated by an electrospinning technique. The morphological and physicochemical properties of scaffolds were studied by scanning electron microscopy (SEM) and spectroscopy. The measurements of tensile strength and water-contact angles suggested that the incorporation of BG into the nanofibers improves the mechanical properties and hydrophilicity of the scaffolds. Biomineralization of the nanofibers was evaluated by soaking them in simulated body fluid (SBF), and the formation of hydroxycarbonate apatite (HCA) layer was determined by EDX and FE-SEM. The results showed that BG-containing nanofibers could induce the formation of HCA on the surface of the composite after 14 days of immersion in SBF. In vitro-cell viability of human mesenchymal stromal cells (hMSCs) on nanofibers was assessed by using the MTT assay. The cell-adhesion results showed that hMSCs were viable at variable time points on the chitosan/PEO/BG nanofiber scaffolds. In addition, the presence of BG enhanced the alkaline phosphatase (ALP) activity of hMSCs cultured on composite scaffolds at day 14 compared to that on pure chitosan/PEO scaffolds. Our results suggest that a chitosan/PEO/BG nanofibrous composite could be a potential candidate for application in tissue engineering.

Original language	English
Pages (from-to)	49144-49152
Number of pages	9
Journal	RSC advances
Volume	4
Issue number	90
DOIs	https://doi.org/10.1039/c4ra06761d
Publication status	Published - 1 Jan 2014
Externally published	Yes

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title = "Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering",

abstract = "A novel hybrid nanofibrous scaffold prepared with chitosan [containing 1.2 wt% polyethylene oxide (PEO)] and bioactive glass (BG) was fabricated by an electrospinning technique. The morphological and physicochemical properties of scaffolds were studied by scanning electron microscopy (SEM) and spectroscopy. The measurements of tensile strength and water-contact angles suggested that the incorporation of BG into the nanofibers improves the mechanical properties and hydrophilicity of the scaffolds. Biomineralization of the nanofibers was evaluated by soaking them in simulated body fluid (SBF), and the formation of hydroxycarbonate apatite (HCA) layer was determined by EDX and FE-SEM. The results showed that BG-containing nanofibers could induce the formation of HCA on the surface of the composite after 14 days of immersion in SBF. In vitro-cell viability of human mesenchymal stromal cells (hMSCs) on nanofibers was assessed by using the MTT assay. The cell-adhesion results showed that hMSCs were viable at variable time points on the chitosan/PEO/BG nanofiber scaffolds. In addition, the presence of BG enhanced the alkaline phosphatase (ALP) activity of hMSCs cultured on composite scaffolds at day 14 compared to that on pure chitosan/PEO scaffolds. Our results suggest that a chitosan/PEO/BG nanofibrous composite could be a potential candidate for application in tissue engineering.",

author = "Sepehr Talebian and Mehdi Mehrali and Saktiswaren Mohan and {Balaji Raghavendran}, {Hanumantha Rao} and Mohammad Mehrali and Khanlou, {Hossein Mohammad} and Tunku Kamarul and Afifi, {Amalina Muhammad} and Abass, {Azlina Amir}",

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doi = "10.1039/c4ra06761d",

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T1 - Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering

AU - Talebian, Sepehr

AU - Mehrali, Mehdi

AU - Mohan, Saktiswaren

AU - Balaji Raghavendran, Hanumantha Rao

AU - Mehrali, Mohammad

AU - Khanlou, Hossein Mohammad

AU - Kamarul, Tunku

AU - Afifi, Amalina Muhammad

AU - Abass, Azlina Amir

PY - 2014/1/1

Y1 - 2014/1/1

N2 - A novel hybrid nanofibrous scaffold prepared with chitosan [containing 1.2 wt% polyethylene oxide (PEO)] and bioactive glass (BG) was fabricated by an electrospinning technique. The morphological and physicochemical properties of scaffolds were studied by scanning electron microscopy (SEM) and spectroscopy. The measurements of tensile strength and water-contact angles suggested that the incorporation of BG into the nanofibers improves the mechanical properties and hydrophilicity of the scaffolds. Biomineralization of the nanofibers was evaluated by soaking them in simulated body fluid (SBF), and the formation of hydroxycarbonate apatite (HCA) layer was determined by EDX and FE-SEM. The results showed that BG-containing nanofibers could induce the formation of HCA on the surface of the composite after 14 days of immersion in SBF. In vitro-cell viability of human mesenchymal stromal cells (hMSCs) on nanofibers was assessed by using the MTT assay. The cell-adhesion results showed that hMSCs were viable at variable time points on the chitosan/PEO/BG nanofiber scaffolds. In addition, the presence of BG enhanced the alkaline phosphatase (ALP) activity of hMSCs cultured on composite scaffolds at day 14 compared to that on pure chitosan/PEO scaffolds. Our results suggest that a chitosan/PEO/BG nanofibrous composite could be a potential candidate for application in tissue engineering.

AB - A novel hybrid nanofibrous scaffold prepared with chitosan [containing 1.2 wt% polyethylene oxide (PEO)] and bioactive glass (BG) was fabricated by an electrospinning technique. The morphological and physicochemical properties of scaffolds were studied by scanning electron microscopy (SEM) and spectroscopy. The measurements of tensile strength and water-contact angles suggested that the incorporation of BG into the nanofibers improves the mechanical properties and hydrophilicity of the scaffolds. Biomineralization of the nanofibers was evaluated by soaking them in simulated body fluid (SBF), and the formation of hydroxycarbonate apatite (HCA) layer was determined by EDX and FE-SEM. The results showed that BG-containing nanofibers could induce the formation of HCA on the surface of the composite after 14 days of immersion in SBF. In vitro-cell viability of human mesenchymal stromal cells (hMSCs) on nanofibers was assessed by using the MTT assay. The cell-adhesion results showed that hMSCs were viable at variable time points on the chitosan/PEO/BG nanofiber scaffolds. In addition, the presence of BG enhanced the alkaline phosphatase (ALP) activity of hMSCs cultured on composite scaffolds at day 14 compared to that on pure chitosan/PEO scaffolds. Our results suggest that a chitosan/PEO/BG nanofibrous composite could be a potential candidate for application in tissue engineering.

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Chitosan (PEO)/bioactive glass hybrid nanofibers for bone tissue engineering

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