Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair

Sara C. Neves, Liliana Moreira Teixeira, Lorenzo Moroni, Rui L. Reis, Clemens van Blitterswijk, Natália M. Alves, Hermanus Bernardus Johannes Karperien, João F. Mano

Research output: Contribution to journalArticleAcademicpeer-review

144 Citations (Scopus)

Abstract

Chitosan (CHT)/poly(ɛ-caprolactone) (PCL) blend 3D fiber-mesh scaffolds were studied as possible support structures for articular cartilage tissue (ACT) repair. Micro-fibers were obtained by wet-spinning of three different polymeric solutions: 100:0 (100CHT), 75:25 (75CHT) and 50:50 (50CHT) wt.% CHT/PCL, using a common solvent solution of 100 vol.% of formic acid. Scanning electron microscopy (SEM) analysis showed a homogeneous surface distribution of PCL. PCL was well dispersed throughout the CHT phase as analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. The fibers were folded into cylindrical moulds and underwent a thermal treatment to obtain the scaffolds. μCT analysis revealed an adequate porosity, pore size and interconnectivity for tissue engineering applications. The PCL component led to a higher fiber surface roughness, decreased the scaffolds swelling ratio and increased their compressive mechanical properties. Biological assays were performed after culturing bovine articular chondrocytes up to 21 days. SEM analysis, live-dead and metabolic activity assays showed that cells attached, proliferated, and were metabolically active over all scaffolds formulations. Cartilaginous extracellular matrix (ECM) formation was observed in all formulations. The 75CHT scaffolds supported the most neo-cartilage formation, as demonstrated by an increase in glycosaminoglycan production. In contrast to 100CHT scaffolds, ECM was homogenously deposited on the 75CHT and 50CHT scaffolds. Although mechanical properties of the 50CHT scaffold were better, the 75CHT scaffold facilitated better neo-cartilage formation.
Original languageEnglish
Pages (from-to)1068-1079
JournalBiomaterials
Volume32
Issue number4
DOIs
Publication statusPublished - 2011

Fingerprint

Chitosan
Cartilage
Scaffolds
Repair
formic acid
Electron Scanning Microscopy
Extracellular Matrix
Fibers
Porosity
Differential Scanning Calorimetry
Articular Cartilage
Fourier Transform Infrared Spectroscopy
Tissue Engineering
Chondrocytes
Glycosaminoglycans
Biological Assay
Assays
Fungi
Hot Temperature
Joints

Keywords

  • Scaffold
  • Polycaprolactone
  • Cartilage tissue engineering
  • IR-80626
  • Chitosan
  • METIS-283818

Cite this

Neves, S. C., Moreira Teixeira, L., Moroni, L., Reis, R. L., van Blitterswijk, C., Alves, N. M., ... Mano, J. F. (2011). Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair. Biomaterials, 32(4), 1068-1079. https://doi.org/10.1016/j.biomaterials.2010.09.073
Neves, Sara C. ; Moreira Teixeira, Liliana ; Moroni, Lorenzo ; Reis, Rui L. ; van Blitterswijk, Clemens ; Alves, Natália M. ; Karperien, Hermanus Bernardus Johannes ; Mano, João F. / Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair. In: Biomaterials. 2011 ; Vol. 32, No. 4. pp. 1068-1079.
@article{74ee084fbf1d4931a892c5832398cd33,
title = "Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair",
abstract = "Chitosan (CHT)/poly(ɛ-caprolactone) (PCL) blend 3D fiber-mesh scaffolds were studied as possible support structures for articular cartilage tissue (ACT) repair. Micro-fibers were obtained by wet-spinning of three different polymeric solutions: 100:0 (100CHT), 75:25 (75CHT) and 50:50 (50CHT) wt.{\%} CHT/PCL, using a common solvent solution of 100 vol.{\%} of formic acid. Scanning electron microscopy (SEM) analysis showed a homogeneous surface distribution of PCL. PCL was well dispersed throughout the CHT phase as analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. The fibers were folded into cylindrical moulds and underwent a thermal treatment to obtain the scaffolds. μCT analysis revealed an adequate porosity, pore size and interconnectivity for tissue engineering applications. The PCL component led to a higher fiber surface roughness, decreased the scaffolds swelling ratio and increased their compressive mechanical properties. Biological assays were performed after culturing bovine articular chondrocytes up to 21 days. SEM analysis, live-dead and metabolic activity assays showed that cells attached, proliferated, and were metabolically active over all scaffolds formulations. Cartilaginous extracellular matrix (ECM) formation was observed in all formulations. The 75CHT scaffolds supported the most neo-cartilage formation, as demonstrated by an increase in glycosaminoglycan production. In contrast to 100CHT scaffolds, ECM was homogenously deposited on the 75CHT and 50CHT scaffolds. Although mechanical properties of the 50CHT scaffold were better, the 75CHT scaffold facilitated better neo-cartilage formation.",
keywords = "Scaffold, Polycaprolactone, Cartilage tissue engineering, IR-80626, Chitosan, METIS-283818",
author = "Neves, {Sara C.} and {Moreira Teixeira}, Liliana and Lorenzo Moroni and Reis, {Rui L.} and {van Blitterswijk}, Clemens and Alves, {Nat{\'a}lia M.} and Karperien, {Hermanus Bernardus Johannes} and Mano, {Jo{\~a}o F.}",
year = "2011",
doi = "10.1016/j.biomaterials.2010.09.073",
language = "English",
volume = "32",
pages = "1068--1079",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",
number = "4",

}

Neves, SC, Moreira Teixeira, L, Moroni, L, Reis, RL, van Blitterswijk, C, Alves, NM, Karperien, HBJ & Mano, JF 2011, 'Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair' Biomaterials, vol. 32, no. 4, pp. 1068-1079. https://doi.org/10.1016/j.biomaterials.2010.09.073

Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair. / Neves, Sara C.; Moreira Teixeira, Liliana; Moroni, Lorenzo; Reis, Rui L.; van Blitterswijk, Clemens; Alves, Natália M.; Karperien, Hermanus Bernardus Johannes; Mano, João F.

In: Biomaterials, Vol. 32, No. 4, 2011, p. 1068-1079.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Chitosan/poly(epsilon-caprolactone) blend scaffolds for cartilage repair

AU - Neves, Sara C.

AU - Moreira Teixeira, Liliana

AU - Moroni, Lorenzo

AU - Reis, Rui L.

AU - van Blitterswijk, Clemens

AU - Alves, Natália M.

AU - Karperien, Hermanus Bernardus Johannes

AU - Mano, João F.

PY - 2011

Y1 - 2011

N2 - Chitosan (CHT)/poly(ɛ-caprolactone) (PCL) blend 3D fiber-mesh scaffolds were studied as possible support structures for articular cartilage tissue (ACT) repair. Micro-fibers were obtained by wet-spinning of three different polymeric solutions: 100:0 (100CHT), 75:25 (75CHT) and 50:50 (50CHT) wt.% CHT/PCL, using a common solvent solution of 100 vol.% of formic acid. Scanning electron microscopy (SEM) analysis showed a homogeneous surface distribution of PCL. PCL was well dispersed throughout the CHT phase as analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. The fibers were folded into cylindrical moulds and underwent a thermal treatment to obtain the scaffolds. μCT analysis revealed an adequate porosity, pore size and interconnectivity for tissue engineering applications. The PCL component led to a higher fiber surface roughness, decreased the scaffolds swelling ratio and increased their compressive mechanical properties. Biological assays were performed after culturing bovine articular chondrocytes up to 21 days. SEM analysis, live-dead and metabolic activity assays showed that cells attached, proliferated, and were metabolically active over all scaffolds formulations. Cartilaginous extracellular matrix (ECM) formation was observed in all formulations. The 75CHT scaffolds supported the most neo-cartilage formation, as demonstrated by an increase in glycosaminoglycan production. In contrast to 100CHT scaffolds, ECM was homogenously deposited on the 75CHT and 50CHT scaffolds. Although mechanical properties of the 50CHT scaffold were better, the 75CHT scaffold facilitated better neo-cartilage formation.

AB - Chitosan (CHT)/poly(ɛ-caprolactone) (PCL) blend 3D fiber-mesh scaffolds were studied as possible support structures for articular cartilage tissue (ACT) repair. Micro-fibers were obtained by wet-spinning of three different polymeric solutions: 100:0 (100CHT), 75:25 (75CHT) and 50:50 (50CHT) wt.% CHT/PCL, using a common solvent solution of 100 vol.% of formic acid. Scanning electron microscopy (SEM) analysis showed a homogeneous surface distribution of PCL. PCL was well dispersed throughout the CHT phase as analyzed by differential scanning calorimetry and Fourier transform infrared spectroscopy. The fibers were folded into cylindrical moulds and underwent a thermal treatment to obtain the scaffolds. μCT analysis revealed an adequate porosity, pore size and interconnectivity for tissue engineering applications. The PCL component led to a higher fiber surface roughness, decreased the scaffolds swelling ratio and increased their compressive mechanical properties. Biological assays were performed after culturing bovine articular chondrocytes up to 21 days. SEM analysis, live-dead and metabolic activity assays showed that cells attached, proliferated, and were metabolically active over all scaffolds formulations. Cartilaginous extracellular matrix (ECM) formation was observed in all formulations. The 75CHT scaffolds supported the most neo-cartilage formation, as demonstrated by an increase in glycosaminoglycan production. In contrast to 100CHT scaffolds, ECM was homogenously deposited on the 75CHT and 50CHT scaffolds. Although mechanical properties of the 50CHT scaffold were better, the 75CHT scaffold facilitated better neo-cartilage formation.

KW - Scaffold

KW - Polycaprolactone

KW - Cartilage tissue engineering

KW - IR-80626

KW - Chitosan

KW - METIS-283818

U2 - 10.1016/j.biomaterials.2010.09.073

DO - 10.1016/j.biomaterials.2010.09.073

M3 - Article

VL - 32

SP - 1068

EP - 1079

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 4

ER -