TY - JOUR
T1 - Chromatin Structure in Bands and Interbands of Polytene Chromosomes Imaged by Atomic Force Microscopy
AU - de Grauw, C.J.
AU - Avogadro, A.
AU - van den Heuvel, D.J.
AU - van der Werf, K.O.
AU - Otto, C.
AU - Kraan, Y.
AU - van Hulst, N.F.
AU - Greve, J.
PY - 1998
Y1 - 1998
N2 - Polytene chromosomes from Drosophila melanogaster, observed from squash preparations, and chromosomes from Chironomus thummi thummi, investigated under physiological conditions, are imaged using an Atomic Force Microscope. Various chromatin fiber structures can be observed with high detail in fixed chromosomes and correspond to structures which are also observed in chromosomes of diploid cells. Unfixed chromosomes can be imaged in buffer and show less fiber-like details because of the inherent soft nature of the chromatin material.
AB - Polytene chromosomes from Drosophila melanogaster, observed from squash preparations, and chromosomes from Chironomus thummi thummi, investigated under physiological conditions, are imaged using an Atomic Force Microscope. Various chromatin fiber structures can be observed with high detail in fixed chromosomes and correspond to structures which are also observed in chromosomes of diploid cells. Unfixed chromosomes can be imaged in buffer and show less fiber-like details because of the inherent soft nature of the chromatin material.
U2 - 10.1006/jsbi.1997.3929
DO - 10.1006/jsbi.1997.3929
M3 - Article
VL - 121
SP - 2
EP - 8
JO - Journal of structural biology
JF - Journal of structural biology
SN - 1047-8477
IS - 1
ER -