TY - JOUR
T1 - Clinical Evaluation of a Simple Image Cytometer for CD4 Enumeration on HIV Infected Patients
AU - Li, X.
AU - Li, X.
AU - Breukers, Christian
AU - Ymeti, Aurel
AU - Pattanapanyasat, Kovit
AU - Sukapirom, Kasama
AU - Terstappen, Leonardus Wendelinus Mathias Marie
AU - Greve, Jan
PY - 2010
Y1 - 2010
N2 - Background: Affordable, easy-to-use, and reliable CD4+ T lymphocyte enumeration systems are needed in resource-constrained settings to monitor HIV. -
Methods: A simple image cytometer was used to count fluorescently labeled CD4+ T and CD8+ T lymphocytes from CD3 immunomagnetically selected cells on blood specimens of 460 HIV-1-infected patients in Siriraj Hospital, Bangkok, Thailand. Results were compared with flow cytometry (FCM). -
Results: CD4+ T lymphocyte counts by image cytometer were comparable (R ≥ 0.97) with those by the FACSCount and the FACScan with a bias of 7.3 and 9.1%, respectively. At very low CD4+ T lymphocyte counts (≤50/μl) some over-count outliers were observed by the FACScan and image cytometer when compared with the FACSCount. For CD8 enumeration, the image cytometer showed a good correlation (R = 0.96) and a consistent undercount (~17%) when compared with the FACSCount. -
Conclusions: Evaluation of the image cytometer for CD4 and CD8 enumeration demonstrated comparable results with FCM on a population of HIV-1-infected patients. The image cytometer is a good alternative method for point-of-care settings in resource-constrained countries.
AB - Background: Affordable, easy-to-use, and reliable CD4+ T lymphocyte enumeration systems are needed in resource-constrained settings to monitor HIV. -
Methods: A simple image cytometer was used to count fluorescently labeled CD4+ T and CD8+ T lymphocytes from CD3 immunomagnetically selected cells on blood specimens of 460 HIV-1-infected patients in Siriraj Hospital, Bangkok, Thailand. Results were compared with flow cytometry (FCM). -
Results: CD4+ T lymphocyte counts by image cytometer were comparable (R ≥ 0.97) with those by the FACSCount and the FACScan with a bias of 7.3 and 9.1%, respectively. At very low CD4+ T lymphocyte counts (≤50/μl) some over-count outliers were observed by the FACScan and image cytometer when compared with the FACSCount. For CD8 enumeration, the image cytometer showed a good correlation (R = 0.96) and a consistent undercount (~17%) when compared with the FACSCount. -
Conclusions: Evaluation of the image cytometer for CD4 and CD8 enumeration demonstrated comparable results with FCM on a population of HIV-1-infected patients. The image cytometer is a good alternative method for point-of-care settings in resource-constrained countries.
KW - METIS-269461
KW - IR-72629
U2 - 10.1002/cyto.b.20488
DO - 10.1002/cyto.b.20488
M3 - Article
SN - 1552-4949
VL - 78B
SP - 31
EP - 36
JO - Cytometry. Part B : Clinical cytometry
JF - Cytometry. Part B : Clinical cytometry
IS - 1
ER -