Clinical Evaluation of a Simple Image Cytometer for CD4 Enumeration on HIV Infected Patients

X. Li, X. Li, Christian Breukers, Aurel Ymeti, Kovit Pattanapanyasat, Kasama Sukapirom, Leonardus Wendelinus Mathias Marie Terstappen, Jan Greve

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Background: Affordable, easy-to-use, and reliable CD4+ T lymphocyte enumeration systems are needed in resource-constrained settings to monitor HIV. - Methods: A simple image cytometer was used to count fluorescently labeled CD4+ T and CD8+ T lymphocytes from CD3 immunomagnetically selected cells on blood specimens of 460 HIV-1-infected patients in Siriraj Hospital, Bangkok, Thailand. Results were compared with flow cytometry (FCM). - Results: CD4+ T lymphocyte counts by image cytometer were comparable (R ≥ 0.97) with those by the FACSCount and the FACScan with a bias of 7.3 and 9.1%, respectively. At very low CD4+ T lymphocyte counts (≤50/μl) some over-count outliers were observed by the FACScan and image cytometer when compared with the FACSCount. For CD8 enumeration, the image cytometer showed a good correlation (R = 0.96) and a consistent undercount (~17%) when compared with the FACSCount. - Conclusions: Evaluation of the image cytometer for CD4 and CD8 enumeration demonstrated comparable results with FCM on a population of HIV-1-infected patients. The image cytometer is a good alternative method for point-of-care settings in resource-constrained countries.
Original languageEnglish
Pages (from-to)31-36
JournalCytometry. Part B : Clinical cytometry
Volume78B
Issue number1
DOIs
Publication statusPublished - 2010

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HIV
T-Lymphocytes
CD4 Lymphocyte Count
HIV-1
Flow Cytometry
Point-of-Care Systems
Thailand
Blood Cells
Population

Keywords

  • METIS-269461
  • IR-72629

Cite this

Li, X. ; Li, X. ; Breukers, Christian ; Ymeti, Aurel ; Pattanapanyasat, Kovit ; Sukapirom, Kasama ; Terstappen, Leonardus Wendelinus Mathias Marie ; Greve, Jan. / Clinical Evaluation of a Simple Image Cytometer for CD4 Enumeration on HIV Infected Patients. In: Cytometry. Part B : Clinical cytometry. 2010 ; Vol. 78B, No. 1. pp. 31-36.
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Clinical Evaluation of a Simple Image Cytometer for CD4 Enumeration on HIV Infected Patients. / Li, X.; Li, X.; Breukers, Christian; Ymeti, Aurel; Pattanapanyasat, Kovit; Sukapirom, Kasama; Terstappen, Leonardus Wendelinus Mathias Marie; Greve, Jan.

In: Cytometry. Part B : Clinical cytometry, Vol. 78B, No. 1, 2010, p. 31-36.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Li, X.

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AU - Sukapirom, Kasama

AU - Terstappen, Leonardus Wendelinus Mathias Marie

AU - Greve, Jan

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N2 - Background: Affordable, easy-to-use, and reliable CD4+ T lymphocyte enumeration systems are needed in resource-constrained settings to monitor HIV. - Methods: A simple image cytometer was used to count fluorescently labeled CD4+ T and CD8+ T lymphocytes from CD3 immunomagnetically selected cells on blood specimens of 460 HIV-1-infected patients in Siriraj Hospital, Bangkok, Thailand. Results were compared with flow cytometry (FCM). - Results: CD4+ T lymphocyte counts by image cytometer were comparable (R ≥ 0.97) with those by the FACSCount and the FACScan with a bias of 7.3 and 9.1%, respectively. At very low CD4+ T lymphocyte counts (≤50/μl) some over-count outliers were observed by the FACScan and image cytometer when compared with the FACSCount. For CD8 enumeration, the image cytometer showed a good correlation (R = 0.96) and a consistent undercount (~17%) when compared with the FACSCount. - Conclusions: Evaluation of the image cytometer for CD4 and CD8 enumeration demonstrated comparable results with FCM on a population of HIV-1-infected patients. The image cytometer is a good alternative method for point-of-care settings in resource-constrained countries.

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