Coherent anti-Stokes Raman scattering (CARS) microscopy driving the future of loaded mesoporous silica imaging

A.L. Fussell, Pei Ting Mah, Herman L. Offerhaus, Sanna-Mari Niemi, Jarno Salonen, Helder A. Santos, Clare Strachan

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

This study reports the use of variants of coherent anti-Stokes Raman scattering (CARS) microscopy as a novel method for improved physicochemical characterization of drug-loaded silica particles. Ordered mesoporous silica is a biomaterial that can be loaded to carry a number of biochemicals, including poorly water-soluble drugs, by allowing the incorporation of drug into nanometer-sized pores. In this work, the loading of two poorly water-soluble model drugs, itraconazole and griseofulvin, in MCM-41 silica microparticles is characterized qualitatively, using the novel approach of CARS microscopy, which has advantages over other analytical approaches used to date and is non-destructive, rapid, label free, confocal and has chemical and physical specificity. The study investigated the effect of two solvent-based loading methods, namely immersion and rotary evaporation, and microparticle size on the three-dimensional (3-D) distribution of the two loaded drugs. Additionally, hyperspectral CARS microscopy was used to confirm the amorphous nature of the loaded drugs. Z-stacked CARS microscopy suggested that the drug, but not the loading method or particle size range, affected 3-D drug distribution. Hyperspectral CARS confirmed that the drug loaded in the MCM-41 silica microparticles was in an amorphous form. The results show that CARS microscopy and hyperspectral CARS microscopy can be used to provide further insights into the structural nature of loaded mesoporous silica microparticles as biomaterials.
Original languageEnglish
Pages (from-to)4870-4877
JournalActa biomaterialia
Volume10
Issue number11
DOIs
Publication statusPublished - 2014

Fingerprint

Raman Spectrum Analysis
Silicon Dioxide
Raman scattering
Microscopy
Microscopic examination
Silica
Imaging techniques
Pharmaceutical Preparations
Multicarrier modulation
Biomaterials
Biocompatible Materials
Griseofulvin
Itraconazole
Water
Labels
Immersion
Evaporation
Particle Size
Particle size

Keywords

  • METIS-306433
  • IR-94968

Cite this

Fussell, A.L. ; Mah, Pei Ting ; Offerhaus, Herman L. ; Niemi, Sanna-Mari ; Salonen, Jarno ; Santos, Helder A. ; Strachan, Clare. / Coherent anti-Stokes Raman scattering (CARS) microscopy driving the future of loaded mesoporous silica imaging. In: Acta biomaterialia. 2014 ; Vol. 10, No. 11. pp. 4870-4877.
@article{a849465c26714cfa84f6e3d1ab3db7a0,
title = "Coherent anti-Stokes Raman scattering (CARS) microscopy driving the future of loaded mesoporous silica imaging",
abstract = "This study reports the use of variants of coherent anti-Stokes Raman scattering (CARS) microscopy as a novel method for improved physicochemical characterization of drug-loaded silica particles. Ordered mesoporous silica is a biomaterial that can be loaded to carry a number of biochemicals, including poorly water-soluble drugs, by allowing the incorporation of drug into nanometer-sized pores. In this work, the loading of two poorly water-soluble model drugs, itraconazole and griseofulvin, in MCM-41 silica microparticles is characterized qualitatively, using the novel approach of CARS microscopy, which has advantages over other analytical approaches used to date and is non-destructive, rapid, label free, confocal and has chemical and physical specificity. The study investigated the effect of two solvent-based loading methods, namely immersion and rotary evaporation, and microparticle size on the three-dimensional (3-D) distribution of the two loaded drugs. Additionally, hyperspectral CARS microscopy was used to confirm the amorphous nature of the loaded drugs. Z-stacked CARS microscopy suggested that the drug, but not the loading method or particle size range, affected 3-D drug distribution. Hyperspectral CARS confirmed that the drug loaded in the MCM-41 silica microparticles was in an amorphous form. The results show that CARS microscopy and hyperspectral CARS microscopy can be used to provide further insights into the structural nature of loaded mesoporous silica microparticles as biomaterials.",
keywords = "METIS-306433, IR-94968",
author = "A.L. Fussell and Mah, {Pei Ting} and Offerhaus, {Herman L.} and Sanna-Mari Niemi and Jarno Salonen and Santos, {Helder A.} and Clare Strachan",
year = "2014",
doi = "10.1016/j.actbio.2014.07.021",
language = "English",
volume = "10",
pages = "4870--4877",
journal = "Acta biomaterialia",
issn = "1742-7061",
publisher = "Elsevier",
number = "11",

}

Coherent anti-Stokes Raman scattering (CARS) microscopy driving the future of loaded mesoporous silica imaging. / Fussell, A.L.; Mah, Pei Ting; Offerhaus, Herman L.; Niemi, Sanna-Mari; Salonen, Jarno; Santos, Helder A.; Strachan, Clare.

In: Acta biomaterialia, Vol. 10, No. 11, 2014, p. 4870-4877.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Coherent anti-Stokes Raman scattering (CARS) microscopy driving the future of loaded mesoporous silica imaging

AU - Fussell, A.L.

AU - Mah, Pei Ting

AU - Offerhaus, Herman L.

AU - Niemi, Sanna-Mari

AU - Salonen, Jarno

AU - Santos, Helder A.

AU - Strachan, Clare

PY - 2014

Y1 - 2014

N2 - This study reports the use of variants of coherent anti-Stokes Raman scattering (CARS) microscopy as a novel method for improved physicochemical characterization of drug-loaded silica particles. Ordered mesoporous silica is a biomaterial that can be loaded to carry a number of biochemicals, including poorly water-soluble drugs, by allowing the incorporation of drug into nanometer-sized pores. In this work, the loading of two poorly water-soluble model drugs, itraconazole and griseofulvin, in MCM-41 silica microparticles is characterized qualitatively, using the novel approach of CARS microscopy, which has advantages over other analytical approaches used to date and is non-destructive, rapid, label free, confocal and has chemical and physical specificity. The study investigated the effect of two solvent-based loading methods, namely immersion and rotary evaporation, and microparticle size on the three-dimensional (3-D) distribution of the two loaded drugs. Additionally, hyperspectral CARS microscopy was used to confirm the amorphous nature of the loaded drugs. Z-stacked CARS microscopy suggested that the drug, but not the loading method or particle size range, affected 3-D drug distribution. Hyperspectral CARS confirmed that the drug loaded in the MCM-41 silica microparticles was in an amorphous form. The results show that CARS microscopy and hyperspectral CARS microscopy can be used to provide further insights into the structural nature of loaded mesoporous silica microparticles as biomaterials.

AB - This study reports the use of variants of coherent anti-Stokes Raman scattering (CARS) microscopy as a novel method for improved physicochemical characterization of drug-loaded silica particles. Ordered mesoporous silica is a biomaterial that can be loaded to carry a number of biochemicals, including poorly water-soluble drugs, by allowing the incorporation of drug into nanometer-sized pores. In this work, the loading of two poorly water-soluble model drugs, itraconazole and griseofulvin, in MCM-41 silica microparticles is characterized qualitatively, using the novel approach of CARS microscopy, which has advantages over other analytical approaches used to date and is non-destructive, rapid, label free, confocal and has chemical and physical specificity. The study investigated the effect of two solvent-based loading methods, namely immersion and rotary evaporation, and microparticle size on the three-dimensional (3-D) distribution of the two loaded drugs. Additionally, hyperspectral CARS microscopy was used to confirm the amorphous nature of the loaded drugs. Z-stacked CARS microscopy suggested that the drug, but not the loading method or particle size range, affected 3-D drug distribution. Hyperspectral CARS confirmed that the drug loaded in the MCM-41 silica microparticles was in an amorphous form. The results show that CARS microscopy and hyperspectral CARS microscopy can be used to provide further insights into the structural nature of loaded mesoporous silica microparticles as biomaterials.

KW - METIS-306433

KW - IR-94968

U2 - 10.1016/j.actbio.2014.07.021

DO - 10.1016/j.actbio.2014.07.021

M3 - Article

VL - 10

SP - 4870

EP - 4877

JO - Acta biomaterialia

JF - Acta biomaterialia

SN - 1742-7061

IS - 11

ER -