Comparative analysis of nanosystems’ effects on human endothelial and monocytic cell functions

Jasmin Matuszak, Philipp Dörfler, Stefan Lyer, Harald Unterweger, Maya Juenet, Cédric Chauvierre, Amr Alaarg, Danielle Franke, Gunter Almer, Isabelle Texier, Josbert M. Metselaar, Ruth Prassl, Christoph Alexiou, Harald Mangge, Didier Letourneur, Iwona Cicha*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)


The objective of our work was to investigate the effects of different types of nanoparticles on endothelial (HUVEC) and monocytic cell functions. We prepared and tested 14 different nanosystems comprising liposomes, lipid nanoparticles, polymer, and iron oxide nanoparticles. Some of the tested nanosystems contained targeting, therapeutic, or contrast agent(s). The effect of particles (0–400 µg/mL) on endothelial-monocytic cell interactions in response to TNF-α was investigated using an arterial bifurcation model and dynamic monocyte adhesion assay. Spontaneous HUVEC migration (0–100 µg/mL nanoparticles) and chemotaxis of monocytic cells towards MCP-1 in presence of particles (0–400 µg/mL) were determined using a barrier assay and a modified Boyden chamber assay, respectively. Lipid nanoparticles dose-dependently reduced monocytic cell chemotaxis and adhesion to activated HUVECs. Liposomal nanoparticles had little effect on cell migration, but one formulation induced monocytic cell recruitment by HUVECs under non-uniform shear stress by about 50%. Fucoidan-coated polymer nanoparticles (25–50 µg/mL) inhibited HUVEC migration and monocytic cell chemotaxis, and had a suppressive effect on monocytic cell recruitment under non-uniform shear stress. No significant effects of iron oxide nanoparticles on monocytic cell recruitment were observed except lauric acid and human albumin-coated particles which increased endothelial-monocytic interactions by 60–70%. Some of the iron oxide nanoparticles inhibited HUVEC migration and monocytic cell chemotaxis. These nanoparticle-induced effects are of importance for vascular cell biology and function and must be considered before the potential clinical use of some of the analyzed nanosystems in cardiovascular applications.

Original languageEnglish
Pages (from-to)957-974
Number of pages18
Issue number9
Publication statusPublished - 21 Oct 2018


  • atherothrombosis
  • endothelial migration
  • endothelial-monocyte interactions
  • monocytic cell chemotaxis
  • Nanoparticles
  • n/a OA procedure


Dive into the research topics of 'Comparative analysis of nanosystems’ effects on human endothelial and monocytic cell functions'. Together they form a unique fingerprint.

Cite this