TY - JOUR
T1 - Comparison of two methods for enumerating circulating tumor cells in carcinoma patients
AU - Balic, Marija
AU - Dandachi, Nadia
AU - Hofmann, Günter
AU - Samonigg, Hellmut
AU - Loibner, Hans
AU - Obwaller, Andreas
AU - Van Der Kooi, Alexander
AU - Tibbe, Arjan G.J.
AU - Doyle, Gerald V.
AU - Terstappen, Leon W.M.M.
AU - Bauernhofer, Thomas
PY - 2005/11/1
Y1 - 2005/11/1
N2 - Background: Monitoring of circulating tumor cells (CTCs) in blood of carcinoma patients treated with novel compounds may be a measurement of treatment effectiveness. Before it can be used clinically, a reliably method is needed to enumerate CTCs. We compared two methods for CTC enumeration, OnkoQuick and the CellSearch system. Methods: We drew 22.5 ml of blood into three CellSave tubes from 15 healthy donors and 61 patients with metastatic carcinoma. After pooling, 15 ml was processed with OncoQuick and 7.5 ml with CellSearch. Results: With both methods no CTCs were found in healthy donors. At least one CTC was detected in 14 of 61 patients (23%) with OncoQuick and 33 of 61 patients (54%) witli CelDSearch (P < 0.0001). The number of CTCs detected was larger for CellSearch (mean 20 CTCs/7.5 ml of blood) than for OncoQuick (3 CTCs/7.5 ml; P < 0.0001). Conclusion: The CellSearch system is a more accurate and sensitive method to enumerate CTCs. Further studies are warranted to evaluate CTC enumeration by the CellSearch system as a monitoring tool for the evaluation of the efficacy of novel anticancer agents.
AB - Background: Monitoring of circulating tumor cells (CTCs) in blood of carcinoma patients treated with novel compounds may be a measurement of treatment effectiveness. Before it can be used clinically, a reliably method is needed to enumerate CTCs. We compared two methods for CTC enumeration, OnkoQuick and the CellSearch system. Methods: We drew 22.5 ml of blood into three CellSave tubes from 15 healthy donors and 61 patients with metastatic carcinoma. After pooling, 15 ml was processed with OncoQuick and 7.5 ml with CellSearch. Results: With both methods no CTCs were found in healthy donors. At least one CTC was detected in 14 of 61 patients (23%) with OncoQuick and 33 of 61 patients (54%) witli CelDSearch (P < 0.0001). The number of CTCs detected was larger for CellSearch (mean 20 CTCs/7.5 ml of blood) than for OncoQuick (3 CTCs/7.5 ml; P < 0.0001). Conclusion: The CellSearch system is a more accurate and sensitive method to enumerate CTCs. Further studies are warranted to evaluate CTC enumeration by the CellSearch system as a monitoring tool for the evaluation of the efficacy of novel anticancer agents.
KW - Circulating tumor cells
KW - Minimal residual disease
KW - Tumor cell enrichment, peripheral blood
UR - http://www.scopus.com/inward/record.url?scp=27644498031&partnerID=8YFLogxK
U2 - 10.1002/cyto.b.20065
DO - 10.1002/cyto.b.20065
M3 - Article
C2 - 16142788
AN - SCOPUS:27644498031
SN - 1552-4949
VL - 68
SP - 25
EP - 30
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
IS - 1
ER -
