TY - JOUR
T1 - Competitive immunological detection of progesterone by means of the ion-step induced response of an immunoFET
AU - Schasfoort, Richardus B.M.
AU - Keldermans, C.E.J.M.
AU - Kooyman, R.P.H.
AU - Bergveld, Piet
AU - Greve, Jan
PY - 1990
Y1 - 1990
N2 - The ion-step method was originally designed to measure immunoreactions via the change in charge density which occurs in an antibody loaded membrane, deposited on an ISFET, upon reaction with a charged antigen. In this paper we show the possibility of detecting an immunoreaction involving a non-charged antigen, progesterone, by using competitive binding of progesterone and a charged progesterone-lysozyme conjugate in the membrane. Both the immunoreaction and the non-specific binding could unequivocally be measured. The detection limit was approximately 10−8 M of progesterone in the sample solution. This proves that the ion-step method is suitable for detection of both charged and non-charged antigens.
AB - The ion-step method was originally designed to measure immunoreactions via the change in charge density which occurs in an antibody loaded membrane, deposited on an ISFET, upon reaction with a charged antigen. In this paper we show the possibility of detecting an immunoreaction involving a non-charged antigen, progesterone, by using competitive binding of progesterone and a charged progesterone-lysozyme conjugate in the membrane. Both the immunoreaction and the non-specific binding could unequivocally be measured. The detection limit was approximately 10−8 M of progesterone in the sample solution. This proves that the ion-step method is suitable for detection of both charged and non-charged antigens.
KW - METIS-129414
KW - IR-72673
U2 - 10.1016/0925-4005(90)80232-O
DO - 10.1016/0925-4005(90)80232-O
M3 - Article
VL - 1
SP - 368
EP - 372
JO - Sensors and actuators. B: Chemical
JF - Sensors and actuators. B: Chemical
SN - 0925-4005
IS - 1-6
ER -