Complement proteins regulating macrophage polarisation on biomaterials

N. Araújo-Gomes, F. Romero-Gavilán*, Y. Zhang, C. Martinez-Ramos, F. Elortza, M. Azkargorta, J. J. Martín de Llano, M. Gurruchaga, I. Goñi, J. J.J.P. van den Beucken, J. Suay

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organically-modified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that greater GPTMS surface concentrations induced greater secretion of TNF-α and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP)and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentration-dependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization.

Original languageEnglish
Pages (from-to)125-133
Number of pages9
JournalColloids and surfaces B: Biointerfaces
Volume181
DOIs
Publication statusPublished - 1 Sep 2019
Externally publishedYes

Keywords

  • Complement system
  • Dental implants
  • Hybrid sol-gel
  • Immune response
  • Macrophage plasticity
  • Proteomics

Fingerprint

Dive into the research topics of 'Complement proteins regulating macrophage polarisation on biomaterials'. Together they form a unique fingerprint.

Cite this