Computational optimization of phase shaped CARS

A.C.W. van Rhijn, A. Jafar Pour, Jennifer Lynn Herek, Herman L. Offerhaus

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

We explore strategies for optimizing selectivity, specificity, and sensitivity in broadband CARS by precalculating pulse shapes using an evolutionary algorithm. We show the possibility of selective excitation of a single constituent in a test case of a mixture of five resonant compounds. The obtainable contrast ratio for a test case of PMMA in a mixture of five resonant compounds is predicted to be 2000:1, and is related the uniqueness of the complex vibrational response of the compound of interest compared to that of the surrounding molecules. Furthermore we investigate how the effects of homodyne mixing in the focal volume affect the obtainable contrast ratio and how noise affects the optimization. We also show preliminary results of experimental optimization of the CARS signal from PMMA microspheres, resulting in high contrast imaging, free of non-resonant background signal.
Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XII
Subtitle of host publication22–24 January 2012, San Francisco, California, United States
EditorsAmmasi Periyasami, Karsten König, Peter T.C. So
Place of PublicationBellingham, WA
DOIs
Publication statusPublished - 22 Jan 2012
EventSPIE BiOS 2012 - The Moscone Center, San Francisco, United States
Duration: 21 Jan 201226 Jan 2012

Publication series

NameProceedings of SPIE
PublisherSPIE
Volume8226
ISSN (Print)0277-786X

Conference

ConferenceSPIE BiOS 2012
CountryUnited States
CitySan Francisco
Period21/01/1226/01/12

Fingerprint

optimization
uniqueness
selectivity
broadband
sensitivity
pulses
excitation
molecules

Keywords

  • METIS-286033
  • IR-84663

Cite this

van Rhijn, A. C. W., Jafar Pour, A., Herek, J. L., & Offerhaus, H. L. (2012). Computational optimization of phase shaped CARS. In A. Periyasami, K. König, & P. T. C. So (Eds.), Multiphoton Microscopy in the Biomedical Sciences XII: 22–24 January 2012, San Francisco, California, United States [82262B] (Proceedings of SPIE; Vol. 8226). Bellingham, WA. https://doi.org/10.1117/12.906630
van Rhijn, A.C.W. ; Jafar Pour, A. ; Herek, Jennifer Lynn ; Offerhaus, Herman L. / Computational optimization of phase shaped CARS. Multiphoton Microscopy in the Biomedical Sciences XII: 22–24 January 2012, San Francisco, California, United States. editor / Ammasi Periyasami ; Karsten König ; Peter T.C. So. Bellingham, WA, 2012. (Proceedings of SPIE).
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abstract = "We explore strategies for optimizing selectivity, specificity, and sensitivity in broadband CARS by precalculating pulse shapes using an evolutionary algorithm. We show the possibility of selective excitation of a single constituent in a test case of a mixture of five resonant compounds. The obtainable contrast ratio for a test case of PMMA in a mixture of five resonant compounds is predicted to be 2000:1, and is related the uniqueness of the complex vibrational response of the compound of interest compared to that of the surrounding molecules. Furthermore we investigate how the effects of homodyne mixing in the focal volume affect the obtainable contrast ratio and how noise affects the optimization. We also show preliminary results of experimental optimization of the CARS signal from PMMA microspheres, resulting in high contrast imaging, free of non-resonant background signal.",
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van Rhijn, ACW, Jafar Pour, A, Herek, JL & Offerhaus, HL 2012, Computational optimization of phase shaped CARS. in A Periyasami, K König & PTC So (eds), Multiphoton Microscopy in the Biomedical Sciences XII: 22–24 January 2012, San Francisco, California, United States., 82262B, Proceedings of SPIE, vol. 8226, Bellingham, WA, SPIE BiOS 2012, San Francisco, United States, 21/01/12. https://doi.org/10.1117/12.906630

Computational optimization of phase shaped CARS. / van Rhijn, A.C.W.; Jafar Pour, A.; Herek, Jennifer Lynn; Offerhaus, Herman L.

Multiphoton Microscopy in the Biomedical Sciences XII: 22–24 January 2012, San Francisco, California, United States. ed. / Ammasi Periyasami; Karsten König; Peter T.C. So. Bellingham, WA, 2012. 82262B (Proceedings of SPIE; Vol. 8226).

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

TY - GEN

T1 - Computational optimization of phase shaped CARS

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Y1 - 2012/1/22

N2 - We explore strategies for optimizing selectivity, specificity, and sensitivity in broadband CARS by precalculating pulse shapes using an evolutionary algorithm. We show the possibility of selective excitation of a single constituent in a test case of a mixture of five resonant compounds. The obtainable contrast ratio for a test case of PMMA in a mixture of five resonant compounds is predicted to be 2000:1, and is related the uniqueness of the complex vibrational response of the compound of interest compared to that of the surrounding molecules. Furthermore we investigate how the effects of homodyne mixing in the focal volume affect the obtainable contrast ratio and how noise affects the optimization. We also show preliminary results of experimental optimization of the CARS signal from PMMA microspheres, resulting in high contrast imaging, free of non-resonant background signal.

AB - We explore strategies for optimizing selectivity, specificity, and sensitivity in broadband CARS by precalculating pulse shapes using an evolutionary algorithm. We show the possibility of selective excitation of a single constituent in a test case of a mixture of five resonant compounds. The obtainable contrast ratio for a test case of PMMA in a mixture of five resonant compounds is predicted to be 2000:1, and is related the uniqueness of the complex vibrational response of the compound of interest compared to that of the surrounding molecules. Furthermore we investigate how the effects of homodyne mixing in the focal volume affect the obtainable contrast ratio and how noise affects the optimization. We also show preliminary results of experimental optimization of the CARS signal from PMMA microspheres, resulting in high contrast imaging, free of non-resonant background signal.

KW - METIS-286033

KW - IR-84663

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SN - 9780819488695

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van Rhijn ACW, Jafar Pour A, Herek JL, Offerhaus HL. Computational optimization of phase shaped CARS. In Periyasami A, König K, So PTC, editors, Multiphoton Microscopy in the Biomedical Sciences XII: 22–24 January 2012, San Francisco, California, United States. Bellingham, WA. 2012. 82262B. (Proceedings of SPIE). https://doi.org/10.1117/12.906630