Computational optimization of phase shaped CARS

A.C.W. van Rhijn, A. Jafar Pour, Jennifer Lynn Herek, Herman L. Offerhaus

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We explore strategies for optimizing selectivity, specificity, and sensitivity in broadband CARS by precalculating pulse shapes using an evolutionary algorithm. We show the possibility of selective excitation of a single constituent in a test case of a mixture of five resonant compounds. The obtainable contrast ratio for a test case of PMMA in a mixture of five resonant compounds is predicted to be 2000:1, and is related the uniqueness of the complex vibrational response of the compound of interest compared to that of the surrounding molecules. Furthermore we investigate how the effects of homodyne mixing in the focal volume affect the obtainable contrast ratio and how noise affects the optimization. We also show preliminary results of experimental optimization of the CARS signal from PMMA microspheres, resulting in high contrast imaging, free of non-resonant background signal.
Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XII
Subtitle of host publication22–24 January 2012, San Francisco, California, United States
EditorsAmmasi Periyasami, Karsten König, Peter T.C. So
Place of PublicationBellingham, WA
Publication statusPublished - 22 Jan 2012
EventSPIE BiOS 2012 - The Moscone Center, San Francisco, United States
Duration: 21 Jan 201226 Jan 2012

Publication series

NameProceedings of SPIE
ISSN (Print)0277-786X


ConferenceSPIE BiOS 2012
Country/TerritoryUnited States
CitySan Francisco


  • METIS-286033
  • IR-84663


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