TY - JOUR
T1 - Conditional immortalization of human atrial myocytes for the generation of in vitro models of atrial fibrillation
AU - Harlaar, Niels
AU - Dekker, Sven O.
AU - Zhang, Juan
AU - Snabel, Rebecca R.
AU - Veldkamp, Marieke W.
AU - Verkerk, Arie O.
AU - Fabres, Carla Cofino
AU - Schwach, Verena
AU - Lerink, Lente J. S.
AU - Rivaud, Mathilde R.
AU - Mulder, Aat A.
AU - Corver, Willem E.
AU - Goumans, Marie Jose T. H.
AU - Dobrev, Dobromir
AU - Klautz, Robert J. M.
AU - Schalij, Martin J.
AU - Veenstra, Gert Jan C.
AU - Passier, Robert
AU - van Brakel, Thomas J.
AU - Pijnappels, Daniel A.
AU - de Vries, Antoine A. F.
PY - 2022/4
Y1 - 2022/4
N2 - The lack of a scalable and robust source of well-differentiated human atrial myocytes constrains the development of in vitro models of atrial fibrillation (AF). Here we show that fully functional atrial myocytes can be generated and expanded one-quadrillion-fold via a conditional cell-immortalization method relying on lentiviral vectors and the doxycycline-controlled expression of a recombinant viral oncogene in human foetal atrial myocytes, and that the immortalized cells can be used to generate in vitro models of AF. The method generated 15 monoclonal cell lines with molecular, cellular and electrophysiological properties resembling those of primary atrial myocytes. Multicellular in vitro models of AF generated using the immortalized atrial myocytes displayed fibrillatory activity (with activation frequencies of 6–8 Hz, consistent with the clinical manifestation of AF), which could be terminated by the administration of clinically approved antiarrhythmic drugs. The conditional cell-immortalization method could be used to generate functional cell lines from other human parenchymal cells, for the development of in vitro models of human disease.
AB - The lack of a scalable and robust source of well-differentiated human atrial myocytes constrains the development of in vitro models of atrial fibrillation (AF). Here we show that fully functional atrial myocytes can be generated and expanded one-quadrillion-fold via a conditional cell-immortalization method relying on lentiviral vectors and the doxycycline-controlled expression of a recombinant viral oncogene in human foetal atrial myocytes, and that the immortalized cells can be used to generate in vitro models of AF. The method generated 15 monoclonal cell lines with molecular, cellular and electrophysiological properties resembling those of primary atrial myocytes. Multicellular in vitro models of AF generated using the immortalized atrial myocytes displayed fibrillatory activity (with activation frequencies of 6–8 Hz, consistent with the clinical manifestation of AF), which could be terminated by the administration of clinically approved antiarrhythmic drugs. The conditional cell-immortalization method could be used to generate functional cell lines from other human parenchymal cells, for the development of in vitro models of human disease.
KW - 22/2 OA procedure
U2 - 10.1038/s41551-021-00827-5
DO - 10.1038/s41551-021-00827-5
M3 - Article
SN - 2157-846X
VL - 6
SP - 389
EP - 402
JO - Nature Biomedical Engineering
JF - Nature Biomedical Engineering
ER -