TY - JOUR
T1 - Confining the Sol-Gel Reaction at the Water/Oil Interface
T2 - Creating Compartmentalized Enzymatic Nano-Organelles for Artificial Cells
AU - Gonçalves, Jenifer Pendiuk
AU - Promlok, Duangkamol
AU - Ivanov, Tsvetomir
AU - Tao, Shijia
AU - Rheinberger, Timo
AU - Jo, Seong Min
AU - Yu, Yingjie
AU - Graf, Robert
AU - Wagner, Manfred
AU - Crespy, Daniel
AU - Wurm, Frederik R.
AU - Caire da Silva, Lucas
AU - Jiang, Shuai
AU - Landfester, Katharina
N1 - Funding Information:
J.P.G. thanks to CAPES (PDSE international PhD exchange program, process number 88881.188473/2018‐01) for the fellowship. This work is part of the research conducted within the Max Planck Consortium for Synthetic Biology (MaxSynBio) jointly funded by the Federal Ministry of Education and Research of Germany (BMBF) and the Max Planck Society. Open Access funding enabled and organized by Projekt DEAL.
Publisher Copyright:
© 2022 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH.
PY - 2023/3/6
Y1 - 2023/3/6
N2 - Living organisms compartmentalize their catalytic reactions in membranes for increased efficiency and selectivity. To mimic the organelles of eukaryotic cells, we develop a mild approach for in situ encapsulating enzymes in aqueous-core silica nanocapsules. In order to confine the sol-gel reaction at the water/oil interface of miniemulsion, we introduce an aminosilane to the silica precursors, which serves as both catalyst and an amphiphilic anchor that electrostatically assembles with negatively charged hydrolyzed alkoxysilanes at the interface. The semi-permeable shell protects enzymes from proteolytic attack, and allows the transport of reactants and products. The enzyme-carrying nanocapsules, as synthetic nano-organelles, are able to perform cascade reactions when enveloped in a polymer vesicle, mimicking the hierarchically compartmentalized reactions in eukaryotic cells. This in situ encapsulation approach provides a versatile platform for the delivery of biomacromolecules.
AB - Living organisms compartmentalize their catalytic reactions in membranes for increased efficiency and selectivity. To mimic the organelles of eukaryotic cells, we develop a mild approach for in situ encapsulating enzymes in aqueous-core silica nanocapsules. In order to confine the sol-gel reaction at the water/oil interface of miniemulsion, we introduce an aminosilane to the silica precursors, which serves as both catalyst and an amphiphilic anchor that electrostatically assembles with negatively charged hydrolyzed alkoxysilanes at the interface. The semi-permeable shell protects enzymes from proteolytic attack, and allows the transport of reactants and products. The enzyme-carrying nanocapsules, as synthetic nano-organelles, are able to perform cascade reactions when enveloped in a polymer vesicle, mimicking the hierarchically compartmentalized reactions in eukaryotic cells. This in situ encapsulation approach provides a versatile platform for the delivery of biomacromolecules.
KW - Artificial Cell
KW - Enzyme Encapsulation
KW - Hollow Silica Nanoparticle
KW - Nanoreactor
KW - Polymer Vesicle
UR - http://www.scopus.com/inward/record.url?scp=85147353147&partnerID=8YFLogxK
U2 - 10.1002/anie.202216966
DO - 10.1002/anie.202216966
M3 - Article
C2 - 36517933
AN - SCOPUS:85147353147
SN - 1433-7851
VL - 62
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 11
M1 - e202216966
ER -