Confocal restricted-height imaging of suspension cells (CRISC) in a PDMS microdevice during apoptosis

Cristina Munoz-Pinedo, Douglas R. Green, Albert van den Berg

    Research output: Contribution to journalArticleAcademicpeer-review

    28 Citations (Scopus)

    Abstract

    We have monitored and imaged cell death induced in human leukemic U937 cells over time using three-color confocal imaging. Three different apoptotic inducers, anti-Fas, TNF-α and Etoposide were used. Individual cascaded events such as loss of mitochondrial transmembrane potential, exposure of phosphatidyl-serine, membrane blebbing and permeabilization of the cell membrane have been observed in real time with different individual cells. From the results, an interesting heterogeneicity in the apoptotic phenotype has been observed. The CRISC method is easy to use and provides biologist with a powerful additional tool to study in real-time processes of several hours of duration such as apoptosis. We predict that the period of cell viability obtained after protein coating of the PDMS devices (>80 h) will also allow monitoring of other biological processes of longer duration or long onset time, such as mitosis, phagocytosis and differentiation.
    Original languageUndefined
    Pages (from-to)628-633
    Number of pages6
    JournalLab on a chip
    Volume5
    Issue number5
    DOIs
    Publication statusPublished - 2005

    Keywords

    • EWI-18364
    • IR-59477
    • METIS-224139

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