Continuous focusing, fractionation and extraction of anionic analytes in a microfluidic chip

Vasileios A. Papadimitriou*, Loes I. Segerink, Jan C.T. Eijkel

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

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    Abstract

    Electrokinetic focusing and separation methods, specifically ion concentration polarization focusing (ICPF), provide a very powerful and easy to use analytical tool for several scientific fields. Nevertheless, the concentrated and separated analytes are effectively trapped inside the chip in picoliter volumes. In this article we propose an ICPF device that allows continuous and selective extraction of the focused analytes. A theoretical background is presented to understand the dynamics of the system and a 1D model was developed that describes the general behavior of the system. We demonstrate the selective extraction of three fluorescent model anionic analytes and we report selective extraction of the analytes at a 300-fold increased concentration.

    Original languageEnglish
    Pages (from-to)3238-3248
    Number of pages11
    JournalLab on a chip
    Volume19
    Issue number19
    DOIs
    Publication statusPublished - 2 Sep 2019

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    Microfluidics
    Fractionation
    Ions
    Polarization
    Equipment and Supplies

    Cite this

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    title = "Continuous focusing, fractionation and extraction of anionic analytes in a microfluidic chip",
    abstract = "Electrokinetic focusing and separation methods, specifically ion concentration polarization focusing (ICPF), provide a very powerful and easy to use analytical tool for several scientific fields. Nevertheless, the concentrated and separated analytes are effectively trapped inside the chip in picoliter volumes. In this article we propose an ICPF device that allows continuous and selective extraction of the focused analytes. A theoretical background is presented to understand the dynamics of the system and a 1D model was developed that describes the general behavior of the system. We demonstrate the selective extraction of three fluorescent model anionic analytes and we report selective extraction of the analytes at a 300-fold increased concentration.",
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    doi = "10.1039/c9lc00434c",
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    Continuous focusing, fractionation and extraction of anionic analytes in a microfluidic chip. / Papadimitriou, Vasileios A.; Segerink, Loes I.; Eijkel, Jan C.T.

    In: Lab on a chip, Vol. 19, No. 19, 02.09.2019, p. 3238-3248.

    Research output: Contribution to journalArticleAcademicpeer-review

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    T1 - Continuous focusing, fractionation and extraction of anionic analytes in a microfluidic chip

    AU - Papadimitriou, Vasileios A.

    AU - Segerink, Loes I.

    AU - Eijkel, Jan C.T.

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    AB - Electrokinetic focusing and separation methods, specifically ion concentration polarization focusing (ICPF), provide a very powerful and easy to use analytical tool for several scientific fields. Nevertheless, the concentrated and separated analytes are effectively trapped inside the chip in picoliter volumes. In this article we propose an ICPF device that allows continuous and selective extraction of the focused analytes. A theoretical background is presented to understand the dynamics of the system and a 1D model was developed that describes the general behavior of the system. We demonstrate the selective extraction of three fluorescent model anionic analytes and we report selective extraction of the analytes at a 300-fold increased concentration.

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