We report the production of bicomponent Janus filaments of miscible aqueous fluids in a micro- fluidic electro-flow focusing device under the action of an AC electric field. The production of liquid filaments can lead to the generation of microfibers by adding a subsequent process of polymerization. Janus microfi- bers are of paramount importance in biomedical appli- cations such as tissue production on crimped scaffolds. We show that the filament length is a function of the frequency signal, voltage amplitude and of the visco- sity and conductivity of the dispersed phase. In par- ticular, Janus filaments with diameters ∼ 10μm and longer than 1 mm are produced by AC voltages with frequencies below 150 kHz and a viscosity of the dis- persed phase ∼ 10 cP.