Controlled cavitation-cell interaction: trans-membrane transport and viability studies

R.J. Dijkink, Severine le Gac, E.H.A. Nijhuis, Albert van den Berg, I. Vermes, Andreas A. Poot, C.D. Ohl

Research output: Contribution to journalArticleAcademicpeer-review

52 Citations (Scopus)


Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.
Original languageEnglish
Pages (from-to)375-390
Number of pages16
JournalPhysics in medicine and biology
Issue number2
Publication statusPublished - 21 Jan 2008


  • EWI-12154
  • METIS-245576
  • IR-59875

Fingerprint Dive into the research topics of 'Controlled cavitation-cell interaction: trans-membrane transport and viability studies'. Together they form a unique fingerprint.

Cite this