Controlled cavitation-cell interaction: trans-membrane transport and viability studies

R.J. Dijkink, Severine le Gac, E.H.A. Nijhuis, Albert van den Berg, I. Vermes, Andreas A. Poot, C.D. Ohl

Research output: Contribution to journalArticleAcademicpeer-review

44 Citations (Scopus)

Abstract

Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.
Original languageUndefined
Pages (from-to)375-390
Number of pages16
JournalPhysics in medicine and biology
Volume53
Issue number2
DOIs
Publication statusPublished - 21 Jan 2008

Keywords

  • EWI-12154
  • METIS-245576
  • IR-59875

Cite this

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title = "Controlled cavitation-cell interaction: trans-membrane transport and viability studies",
abstract = "Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.",
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Controlled cavitation-cell interaction: trans-membrane transport and viability studies. / Dijkink, R.J.; le Gac, Severine; Nijhuis, E.H.A.; van den Berg, Albert; Vermes, I.; Poot, Andreas A.; Ohl, C.D.

In: Physics in medicine and biology, Vol. 53, No. 2, 21.01.2008, p. 375-390.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Controlled cavitation-cell interaction: trans-membrane transport and viability studies

AU - Dijkink, R.J.

AU - le Gac, Severine

AU - Nijhuis, E.H.A.

AU - van den Berg, Albert

AU - Vermes, I.

AU - Poot, Andreas A.

AU - Ohl, C.D.

N1 - 10.1088/0031-9155/53/2/006

PY - 2008/1/21

Y1 - 2008/1/21

N2 - Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.

AB - Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.

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KW - METIS-245576

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M3 - Article

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EP - 390

JO - Physics in medicine and biology

JF - Physics in medicine and biology

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