Abstract
| Original language | Undefined |
|---|---|
| Pages (from-to) | 375-390 |
| Number of pages | 16 |
| Journal | Physics in medicine and biology |
| Volume | 53 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 21 Jan 2008 |
Keywords
- EWI-12154
- METIS-245576
- IR-59875
Cite this
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Controlled cavitation-cell interaction: trans-membrane transport and viability studies. / Dijkink, R.J.; le Gac, Severine; Nijhuis, E.H.A.; van den Berg, Albert; Vermes, I.; Poot, Andreas A.; Ohl, C.D.
In: Physics in medicine and biology, Vol. 53, No. 2, 21.01.2008, p. 375-390.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Controlled cavitation-cell interaction: trans-membrane transport and viability studies
AU - Dijkink, R.J.
AU - le Gac, Severine
AU - Nijhuis, E.H.A.
AU - van den Berg, Albert
AU - Vermes, I.
AU - Poot, Andreas A.
AU - Ohl, C.D.
N1 - 10.1088/0031-9155/53/2/006
PY - 2008/1/21
Y1 - 2008/1/21
N2 - Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.
AB - Cavitation bubble dynamics close to a rigid surface gives rise to a rapid and transient fluid flow. A single bubble is created with a laser pulse at different stand-off distances from the rigid surface, where the stand-off distance γ is defined by γ = h/Rmax, with h being the initial distance and Rmax being the maximum bubble radius. When the surface is covered with adherent cells, molecular delivery and cell detachment after single cavitation activity are observed at different locations. We find a maximum of cell detachment at a normalized stand-off distance of γ ~ 0.65. In contrast, the maximum of the molecular uptake is found when γ approaches 0. The single cavitation event has only little effect on the viability of cells in the non-detached area. We find apoptosis of cells only very close to the area of detachment and, additionally, the metabolism of the non-detached cells shows no pronounced difference compared to control cells according to an MTS assay. Thus, although the cavitation event is responsible for the detachment of cells, only few of the remaining cells undergo a permanent change.
KW - EWI-12154
KW - METIS-245576
KW - IR-59875
U2 - 10.1088/0031-9155/53/2/006
DO - 10.1088/0031-9155/53/2/006
M3 - Article
VL - 53
SP - 375
EP - 390
JO - Physics in medicine and biology
JF - Physics in medicine and biology
SN - 0031-9155
IS - 2
ER -