Controlled integration of polymers into viral capsids

Marta Comellas-Aragones, M. Comellas Aragones, Andres de la Escosura, A (Ton) J. Dirks, A.M. van der Ham, Anne van der Ham, Anna Fuste-Cune, Jeroen Johannes Lambertus Maria Cornelissen, Roeland J.M. Nolte

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

In this paper, we describe the controlled incorporation of two synthetic polymers with different structures in the cowpea chlorotic mottle virus (CCMV) capsid. Poly(ethylene glycol) (PEG) chains have been attached to the amine groups of lysine residues on the outer surface of the viral capsid. The functionalization of CCMV with PEG chains provoked a slow but irreversible dissociation of the virus into PEG-coat protein (CP) subunits, likely due to steric interference between the protein−protein subunits as a result of the presence of the PEG chains. This thermodynamic instability, however, can be overcome if a second polymer, such as polystyrene sulfonate (PSS), is present within the capsid. After complete disassembly of the PEG-CCMV conjugates and removal of the viral RNA, incubation of the PEG-functionalized coat proteins with PSS resulted in the formation of much more robust PSS-CCMV-PEG capsids with a diameter of 18 nm (T = 1 capsids). These are the first virus-like particles bearing synthetic organic polymers both inside and outside the viral capsid, opening a new route to the synthesis of biohybrid nanostructured materials based on viruses
Original languageUndefined
Pages (from-to)3141-3147
Number of pages7
JournalBiomacromolecules
Volume10
DOIs
Publication statusPublished - 2009

Keywords

  • IR-77784
  • METIS-263227

Cite this

Comellas-Aragones, M., Comellas Aragones, M., de la Escosura, A., Dirks, A. T. J., van der Ham, A. M., van der Ham, A., ... Nolte, R. J. M. (2009). Controlled integration of polymers into viral capsids. Biomacromolecules, 10, 3141-3147. https://doi.org/10.1021/bm9007953
Comellas-Aragones, Marta ; Comellas Aragones, M. ; de la Escosura, Andres ; Dirks, A (Ton) J. ; van der Ham, A.M. ; van der Ham, Anne ; Fuste-Cune, Anna ; Cornelissen, Jeroen Johannes Lambertus Maria ; Nolte, Roeland J.M. / Controlled integration of polymers into viral capsids. In: Biomacromolecules. 2009 ; Vol. 10. pp. 3141-3147.
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title = "Controlled integration of polymers into viral capsids",
abstract = "In this paper, we describe the controlled incorporation of two synthetic polymers with different structures in the cowpea chlorotic mottle virus (CCMV) capsid. Poly(ethylene glycol) (PEG) chains have been attached to the amine groups of lysine residues on the outer surface of the viral capsid. The functionalization of CCMV with PEG chains provoked a slow but irreversible dissociation of the virus into PEG-coat protein (CP) subunits, likely due to steric interference between the protein−protein subunits as a result of the presence of the PEG chains. This thermodynamic instability, however, can be overcome if a second polymer, such as polystyrene sulfonate (PSS), is present within the capsid. After complete disassembly of the PEG-CCMV conjugates and removal of the viral RNA, incubation of the PEG-functionalized coat proteins with PSS resulted in the formation of much more robust PSS-CCMV-PEG capsids with a diameter of 18 nm (T = 1 capsids). These are the first virus-like particles bearing synthetic organic polymers both inside and outside the viral capsid, opening a new route to the synthesis of biohybrid nanostructured materials based on viruses",
keywords = "IR-77784, METIS-263227",
author = "Marta Comellas-Aragones and {Comellas Aragones}, M. and {de la Escosura}, Andres and Dirks, {A (Ton) J.} and {van der Ham}, A.M. and {van der Ham}, Anne and Anna Fuste-Cune and Cornelissen, {Jeroen Johannes Lambertus Maria} and Nolte, {Roeland J.M.}",
year = "2009",
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Comellas-Aragones, M, Comellas Aragones, M, de la Escosura, A, Dirks, ATJ, van der Ham, AM, van der Ham, A, Fuste-Cune, A, Cornelissen, JJLM & Nolte, RJM 2009, 'Controlled integration of polymers into viral capsids', Biomacromolecules, vol. 10, pp. 3141-3147. https://doi.org/10.1021/bm9007953

Controlled integration of polymers into viral capsids. / Comellas-Aragones, Marta; Comellas Aragones, M.; de la Escosura, Andres; Dirks, A (Ton) J.; van der Ham, A.M.; van der Ham, Anne; Fuste-Cune, Anna; Cornelissen, Jeroen Johannes Lambertus Maria; Nolte, Roeland J.M.

In: Biomacromolecules, Vol. 10, 2009, p. 3141-3147.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Controlled integration of polymers into viral capsids

AU - Comellas-Aragones, Marta

AU - Comellas Aragones, M.

AU - de la Escosura, Andres

AU - Dirks, A (Ton) J.

AU - van der Ham, A.M.

AU - van der Ham, Anne

AU - Fuste-Cune, Anna

AU - Cornelissen, Jeroen Johannes Lambertus Maria

AU - Nolte, Roeland J.M.

PY - 2009

Y1 - 2009

N2 - In this paper, we describe the controlled incorporation of two synthetic polymers with different structures in the cowpea chlorotic mottle virus (CCMV) capsid. Poly(ethylene glycol) (PEG) chains have been attached to the amine groups of lysine residues on the outer surface of the viral capsid. The functionalization of CCMV with PEG chains provoked a slow but irreversible dissociation of the virus into PEG-coat protein (CP) subunits, likely due to steric interference between the protein−protein subunits as a result of the presence of the PEG chains. This thermodynamic instability, however, can be overcome if a second polymer, such as polystyrene sulfonate (PSS), is present within the capsid. After complete disassembly of the PEG-CCMV conjugates and removal of the viral RNA, incubation of the PEG-functionalized coat proteins with PSS resulted in the formation of much more robust PSS-CCMV-PEG capsids with a diameter of 18 nm (T = 1 capsids). These are the first virus-like particles bearing synthetic organic polymers both inside and outside the viral capsid, opening a new route to the synthesis of biohybrid nanostructured materials based on viruses

AB - In this paper, we describe the controlled incorporation of two synthetic polymers with different structures in the cowpea chlorotic mottle virus (CCMV) capsid. Poly(ethylene glycol) (PEG) chains have been attached to the amine groups of lysine residues on the outer surface of the viral capsid. The functionalization of CCMV with PEG chains provoked a slow but irreversible dissociation of the virus into PEG-coat protein (CP) subunits, likely due to steric interference between the protein−protein subunits as a result of the presence of the PEG chains. This thermodynamic instability, however, can be overcome if a second polymer, such as polystyrene sulfonate (PSS), is present within the capsid. After complete disassembly of the PEG-CCMV conjugates and removal of the viral RNA, incubation of the PEG-functionalized coat proteins with PSS resulted in the formation of much more robust PSS-CCMV-PEG capsids with a diameter of 18 nm (T = 1 capsids). These are the first virus-like particles bearing synthetic organic polymers both inside and outside the viral capsid, opening a new route to the synthesis of biohybrid nanostructured materials based on viruses

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KW - METIS-263227

U2 - 10.1021/bm9007953

DO - 10.1021/bm9007953

M3 - Article

VL - 10

SP - 3141

EP - 3147

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

ER -

Comellas-Aragones M, Comellas Aragones M, de la Escosura A, Dirks ATJ, van der Ham AM, van der Ham A et al. Controlled integration of polymers into viral capsids. Biomacromolecules. 2009;10:3141-3147. https://doi.org/10.1021/bm9007953