Controlled vesicle deformation and lysis by single oscillating bubbles

P.G.M. Marmottant, Sascha Hilgenfeldt

Research output: Contribution to journalArticleAcademicpeer-review

557 Citations (Scopus)

Abstract

The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence1, 2. In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images3. The observation of bubble-enhanced sonoporation4, 5, 6¿acoustically induced rupture of membranes¿has also opened up intriguing possibilities for the therapeutic application of sonoporation as an alternative to cell-wall permeation techniques such as electroporation7 and particle guns8. However, these pioneering experiments have not been able to pinpoint the mechanism by which the violently collapsing bubble opens pores or larger holes in membranes. Here we present an experiment in which gentle (linear) bubble oscillations are sufficient to achieve rupture of lipid membranes. In this regime, the bubble dynamics and the ensuing sonoporation can be accurately controlled. The use of microbubbles as focusing agents makes acoustics on the micrometre scale (microacoustics) a viable tool, with possible applications in cell manipulation and cell-wall permeation as well as in microfluidic devices.
Original languageUndefined
Pages (from-to)153-156
Number of pages4
JournalNature
Volume423
Issue number6936
DOIs
Publication statusPublished - 2003

Keywords

  • IR-40927
  • METIS-216138

Cite this

Marmottant, P.G.M. ; Hilgenfeldt, Sascha. / Controlled vesicle deformation and lysis by single oscillating bubbles. In: Nature. 2003 ; Vol. 423, No. 6936. pp. 153-156.
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Marmottant, PGM & Hilgenfeldt, S 2003, 'Controlled vesicle deformation and lysis by single oscillating bubbles' Nature, vol. 423, no. 6936, pp. 153-156. https://doi.org/10.1038/nature01613

Controlled vesicle deformation and lysis by single oscillating bubbles. / Marmottant, P.G.M.; Hilgenfeldt, Sascha.

In: Nature, Vol. 423, No. 6936, 2003, p. 153-156.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Marmottant, P.G.M.

AU - Hilgenfeldt, Sascha

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AB - The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence1, 2. In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images3. The observation of bubble-enhanced sonoporation4, 5, 6¿acoustically induced rupture of membranes¿has also opened up intriguing possibilities for the therapeutic application of sonoporation as an alternative to cell-wall permeation techniques such as electroporation7 and particle guns8. However, these pioneering experiments have not been able to pinpoint the mechanism by which the violently collapsing bubble opens pores or larger holes in membranes. Here we present an experiment in which gentle (linear) bubble oscillations are sufficient to achieve rupture of lipid membranes. In this regime, the bubble dynamics and the ensuing sonoporation can be accurately controlled. The use of microbubbles as focusing agents makes acoustics on the micrometre scale (microacoustics) a viable tool, with possible applications in cell manipulation and cell-wall permeation as well as in microfluidic devices.

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