Controlling fluorescent proteins by manipulating the local density of photonic states

Christian Blum, Y. Cesa, Johanna M. van den Broek, Allard Mosk, Vinod Subramaniam, Willem L. Vos

Research output: Chapter in Book/Report/Conference proceedingChapterAcademic

1 Citation (Scopus)

Abstract

We present the first demonstration of control of the emission lifetime of a biological emitter by manipulating the local density of optical states (LDOS). LDOS control is achieved by positioning the emitters at defined distances from a metallic mirror. This results in a characteristic oscillation in the fluorescence decay rate. Since only the emitting species contribute to the emission lifetimes, the radiative and nonradiative decay rates derived from the lifetime changes characterize specifically the on- states of the emitter. We have thus experimentally determined the decay rates, and by extension the quantum efficiency and emission oscillator strength, of exclusively the emitting states of the widely used Enhanced Green Fluorescent Protein (EGFP). This approach is in contrast to other methods that average over emitting and dark states. The quantum efficiency of the on-states determined for EGFP is 72%. This value is higher than previously reported values determined by methods that average over on- and off-states, as is expected for this system with known dark states. The method presented is especially interesting for photophysically complex systems like fluorescent proteins, where a range of emitting and dark forms has been observed
Original languageUndefined
Title of host publicationAdvanced Microscopy Techniques
EditorsPaul J. Campagnola, Ernst H.K. Stelzer, Gert von Bally
PublisherSPIE / OSA
Pages73670C
ISBN (Print)9780819476432
DOIs
Publication statusPublished - 2009
EventAdvanced Microscopy Techniques - Munich, Germany
Duration: 14 Jun 200914 Jun 2009

Publication series

NameProceedings of SPIE-OSA Biomedical Optics
PublisherSPIE/OSA
Volume7367
ISSN (Print)1605-7422

Conference

ConferenceAdvanced Microscopy Techniques
Period14/06/0914/06/09
Other14 June 2009

Keywords

  • Fluorescent Proteins
  • fluorescence lifetime
  • IR-77737
  • Photophysics
  • GFP
  • Quantum efficiency
  • LDOS

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