Abstract
A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanisms at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user-friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover, snap freezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests.
In this thesis, starting from understanding the heat transfer mechanisms of different tissue-freezing procedures, an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen is presented. The device can be used directly at the location of tissue acquisition and also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples.
In this thesis, starting from understanding the heat transfer mechanisms of different tissue-freezing procedures, an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen is presented. The device can be used directly at the location of tissue acquisition and also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples.
| Original language | English |
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| Qualification | Doctor of Philosophy |
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| Award date | 16 Jun 2021 |
| Place of Publication | Enschede |
| Publisher | |
| Print ISBNs | 978-90-365-5197-7 |
| DOIs | |
| Publication status | Published - 16 Jun 2021 |