Cooling dynamics in thermal quenching for cryopreservation

Sahil Jagga

Research output: ThesisPhD Thesis - Research UT, graduation UT

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A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanisms at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user-friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover, snap freezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests.

In this thesis, starting from understanding the heat transfer mechanisms of different tissue-freezing procedures, an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen is presented. The device can be used directly at the location of tissue acquisition and also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Twente
  • ter Brake, H.J.M., Supervisor
  • Vanapalli, S., Co-Supervisor
Award date16 Jun 2021
Place of PublicationEnschede
Print ISBNs978-90-365-5197-7
Publication statusPublished - 16 Jun 2021


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