Cooling of a vial in a snapfreezing device without using sacrificial cryogens

Michiel A.J. van Limbeek, Sahil Jagga, Harry Holland, Koen Ledeboer, Marcel ter Brake, Srinivas Vanapalli (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

12 Downloads (Pure)

Abstract

A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanism at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover snapfreezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests. Herein, we report an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen and therefore can be used directly at the location where the tissue is acquired. This device also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples. Cooling experiments of a vial in the snap freezing device show that the cooling rates similar to or faster than quenching in liquid nitrogen are feasible. We performed experiments with several set point conditions and compared the results with a mathematical model.

Original languageEnglish
Article number3510
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 5 Mar 2019

Fingerprint

Freezing
Equipment and Supplies
Nitrogen
Molecular Medicine
Theoretical Models

Cite this

@article{108bdcc1daba4474888fbb1fddf54612,
title = "Cooling of a vial in a snapfreezing device without using sacrificial cryogens",
abstract = "A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanism at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover snapfreezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests. Herein, we report an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen and therefore can be used directly at the location where the tissue is acquired. This device also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples. Cooling experiments of a vial in the snap freezing device show that the cooling rates similar to or faster than quenching in liquid nitrogen are feasible. We performed experiments with several set point conditions and compared the results with a mathematical model.",
author = "{van Limbeek}, {Michiel A.J.} and Sahil Jagga and Harry Holland and Koen Ledeboer and {ter Brake}, Marcel and Srinivas Vanapalli",
year = "2019",
month = "3",
day = "5",
doi = "10.1038/s41598-019-40115-6",
language = "English",
volume = "9",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

Cooling of a vial in a snapfreezing device without using sacrificial cryogens. / van Limbeek, Michiel A.J.; Jagga, Sahil; Holland, Harry; Ledeboer, Koen; ter Brake, Marcel; Vanapalli, Srinivas (Corresponding Author).

In: Scientific reports, Vol. 9, No. 1, 3510, 05.03.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cooling of a vial in a snapfreezing device without using sacrificial cryogens

AU - van Limbeek, Michiel A.J.

AU - Jagga, Sahil

AU - Holland, Harry

AU - Ledeboer, Koen

AU - ter Brake, Marcel

AU - Vanapalli, Srinivas

PY - 2019/3/5

Y1 - 2019/3/5

N2 - A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanism at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover snapfreezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests. Herein, we report an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen and therefore can be used directly at the location where the tissue is acquired. This device also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples. Cooling experiments of a vial in the snap freezing device show that the cooling rates similar to or faster than quenching in liquid nitrogen are feasible. We performed experiments with several set point conditions and compared the results with a mathematical model.

AB - A fresh and frozen high-quality patient bio-sample is required in molecular medicine for the identification of disease-associated mechanism at molecular levels. A common cooling procedure is immersing the tissue enclosed in a vial in a coolant such as liquid nitrogen. This procedure is not user friendly and is laborious as reducing the lag time from excision time to freezing depends on the logistic organizational structure within a hospital. Moreover snapfreezing must be done as soon as possible after tissue excision to preserve the tissue quality for molecular tests. Herein, we report an electrically powered snap freezing device as an alternative to quenching the vial in liquid nitrogen and therefore can be used directly at the location where the tissue is acquired. This device also facilitates the study of the effect of freezing conditions on the various molecular processes in the samples. Cooling experiments of a vial in the snap freezing device show that the cooling rates similar to or faster than quenching in liquid nitrogen are feasible. We performed experiments with several set point conditions and compared the results with a mathematical model.

UR - http://www.scopus.com/inward/record.url?scp=85062587649&partnerID=8YFLogxK

U2 - 10.1038/s41598-019-40115-6

DO - 10.1038/s41598-019-40115-6

M3 - Article

VL - 9

JO - Scientific reports

JF - Scientific reports

SN - 2045-2322

IS - 1

M1 - 3510

ER -