Covalent Coupling of Polyamines to Aminobead-Covered ISFETs

Geert Besselink; Gijs Dirks; Wouter Olthuis; Piet Bergveld

doi:10.1007/978-94-011-5010-1_17

Covalent Coupling of Polyamines to Aminobead-Covered ISFETs

Geert Besselink, Gijs Dirks, Wouter Olthuis, Piet Bergveld

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

Abstract

The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.

Original language	English
Title of host publication	Sensor technology in the Netherlands - state of the art
Subtitle of host publication	proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands
Editors	A. van den Berg, P. Bergveld
Place of Publication	Dordrecht
Publisher	Kluwer Academic Publishers
Pages	111-115
Number of pages	5
ISBN (Electronic)	978-94-011-5010-1
ISBN (Print)	978-94-010-6103-2
DOIs	https://doi.org/10.1007/978-94-011-5010-1_17
Publication status	Published - 2 Mar 1998
Event	Dutch Sensor Conference 1998: Sensor Technology in the Netherlands: State of the Art - University of Twente, Enschede, Netherlands Duration: 2 Mar 1998 → 3 Mar 1998

Publication series

Name	MESA monographs
Publisher	Kluwer Academic Publishers
Volume	2

Conference

Conference	Dutch Sensor Conference 1998
Country	Netherlands
City	Enschede
Period	2/03/98 → 3/03/98

Keywords

ISFET
Modification
Latex
Beads
Polyamine
Heparin

Access to Document

10.1007/978-94-011-5010-1_17

Cite this

Besselink, G., Dirks, G., Olthuis, W., & Bergveld, P. (1998). Covalent Coupling of Polyamines to Aminobead-Covered ISFETs. In A. van den Berg, & P. Bergveld (Eds.), Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands (pp. 111-115). (MESA monographs; Vol. 2). Kluwer Academic Publishers. https://doi.org/10.1007/978-94-011-5010-1_17

Besselink, Geert ; Dirks, Gijs ; Olthuis, Wouter ; Bergveld, Piet. / Covalent Coupling of Polyamines to Aminobead-Covered ISFETs. Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. editor / A. van den Berg ; P. Bergveld. Dordrecht : Kluwer Academic Publishers, 1998. pp. 111-115 (MESA monographs).

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title = "Covalent Coupling of Polyamines to Aminobead-Covered ISFETs",

abstract = "The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.",

keywords = "ISFET, Modification, Latex, Beads, Polyamine, Heparin",

author = "Geert Besselink and Gijs Dirks and Wouter Olthuis and Piet Bergveld",

year = "1998",

month = mar,

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doi = "10.1007/978-94-011-5010-1_17",

language = "English",

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series = "MESA monographs",

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editor = "{van den Berg}, A. and P. Bergveld",

booktitle = "Sensor technology in the Netherlands - state of the art",

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note = "Dutch Sensor Conference 1998 : Sensor Technology in the Netherlands: State of the Art ; Conference date: 02-03-1998 Through 03-03-1998",

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Besselink, G, Dirks, G, Olthuis, W & Bergveld, P 1998, Covalent Coupling of Polyamines to Aminobead-Covered ISFETs. in A van den Berg & P Bergveld (eds), Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. MESA monographs, vol. 2, Kluwer Academic Publishers, Dordrecht, pp. 111-115, Dutch Sensor Conference 1998, Enschede, Netherlands, 2/03/98. https://doi.org/10.1007/978-94-011-5010-1_17

Covalent Coupling of Polyamines to Aminobead-Covered ISFETs. / Besselink, Geert; Dirks, Gijs; Olthuis, Wouter; Bergveld, Piet.

Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. ed. / A. van den Berg; P. Bergveld. Dordrecht : Kluwer Academic Publishers, 1998. p. 111-115 (MESA monographs; Vol. 2).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Covalent Coupling of Polyamines to Aminobead-Covered ISFETs

AU - Besselink, Geert

AU - Dirks, Gijs

AU - Olthuis, Wouter

AU - Bergveld, Piet

PY - 1998/3/2

Y1 - 1998/3/2

N2 - The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.

AB - The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.

KW - ISFET

KW - Modification

KW - Latex

KW - Beads

KW - Polyamine

KW - Heparin

U2 - 10.1007/978-94-011-5010-1_17

DO - 10.1007/978-94-011-5010-1_17

M3 - Conference contribution

SN - 978-94-010-6103-2

T3 - MESA monographs

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EP - 115

BT - Sensor technology in the Netherlands - state of the art

A2 - van den Berg, A.

A2 - Bergveld, P.

PB - Kluwer Academic Publishers

CY - Dordrecht

T2 - Dutch Sensor Conference 1998

Y2 - 2 March 1998 through 3 March 1998

ER -

Besselink G, Dirks G, Olthuis W, Bergveld P. Covalent Coupling of Polyamines to Aminobead-Covered ISFETs. In van den Berg A, Bergveld P, editors, Sensor technology in the Netherlands - state of the art: proceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands. Dordrecht: Kluwer Academic Publishers. 1998. p. 111-115. (MESA monographs). https://doi.org/10.1007/978-94-011-5010-1_17

Covalent Coupling of Polyamines to Aminobead-Covered ISFETs

Abstract

Publication series

Conference

Keywords

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