@inproceedings{460d86491e4a490cab544d955d67093a,
title = "Covalent Coupling of Polyamines to Aminobead-Covered ISFETs",
abstract = "The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.",
keywords = "ISFET, Modification, Latex, Beads, Polyamine, Heparin",
author = "Geert Besselink and Gijs Dirks and Wouter Olthuis and Piet Bergveld",
year = "1998",
month = mar,
day = "2",
doi = "10.1007/978-94-011-5010-1_17",
language = "English",
isbn = "978-94-010-6103-2",
series = "MESA monographs",
publisher = "Kluwer Academic Publishers",
pages = "111--115",
editor = "{van den Berg}, A. and P. Bergveld",
booktitle = "Sensor technology in the Netherlands - state of the art",
address = "Netherlands",
note = "Dutch Sensor Conference 1998 : Sensor Technology in the Netherlands: State of the Art ; Conference date: 02-03-1998 Through 03-03-1998",
}