The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.
|Publisher||Kluwer Academic Publishers|
|Conference||Dutch Sensor Conference 1998|
|Period||2/03/98 → 3/03/98|