Covalent Coupling of Polyamines to Aminobead-Covered ISFETs

Geert Besselink, Gijs Dirks, Wouter Olthuis, Piet Bergveld

    Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review


    The so-called ion-step method is a novel potentiometric approach for determination of protein concentration, in which modified ISFETs are used [1, 2]. Aim of our current research is to develop a generic technology for immobilization of proteins (and other biomolecules) to the ISFET gate in order to confer specific binding properties to the ISFET. In this report, a new modification procedure is introduced, namely the covering of the ISFET surface with a monolayerof latex beads followed by the covalentcoupling of (poly)amines to these beads. The resulting ISFET devices could be used for detection of heparin in the concentration range of 0.025–0.75 U/ml. Considerable differences were found concerning the sensitivity of heparin detection when employing different types of polyamine ligands.
    Original languageEnglish
    Title of host publicationSensor technology in the Netherlands - state of the art
    Subtitle of host publicationproceedings of the Dutch Sensor Conference, 2-3 March, 1998, University of Twente, the Netherlands
    EditorsA. van den Berg, P. Bergveld
    Place of PublicationDordrecht
    PublisherKluwer Academic Publishers
    Number of pages5
    ISBN (Electronic)978-94-011-5010-1
    ISBN (Print)978-94-010-6103-2
    Publication statusPublished - 2 Mar 1998
    EventDutch Sensor Conference 1998: Sensor Technology in the Netherlands: State of the Art - University of Twente, Enschede, Netherlands
    Duration: 2 Mar 19983 Mar 1998

    Publication series

    NameMESA monographs
    PublisherKluwer Academic Publishers


    ConferenceDutch Sensor Conference 1998


    • ISFET
    • Modification
    • Latex
    • Beads
    • Polyamine
    • Heparin


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