Covalent microcontact printing of proteins for cell patterning

D.I. Rozkiewicz, Yvonne M. Kraan, Marc W.T. Werten, Frits A. de Wolf, Vinod Subramaniam, B.J. Ravoo, David Reinhoudt

Research output: Contribution to journalArticleAcademicpeer-review

99 Citations (Scopus)

Abstract

We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive islands, the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.
Original languageEnglish
Pages (from-to)6290-6297
Number of pages8
JournalChemistry: a European journal
Volume12
Issue number24
DOIs
Publication statusPublished - 2006

Fingerprint

Printing
Proteins
Polyethylene glycols
Monolayers
Cell adhesion
Aldehydes
Substrates
Imines
Self assembled monolayers
Polydimethylsiloxane
Surface chemistry
Collagen
Gold
Adhesives
Glass

Keywords

  • METIS-236491
  • immobilization
  • PROTEINS
  • Surface chemistry
  • Microcontact Printing
  • IR-72055
  • Cell adhesion

Cite this

Rozkiewicz, D. I., Kraan, Y. M., Werten, M. W. T., de Wolf, F. A., Subramaniam, V., Ravoo, B. J., & Reinhoudt, D. (2006). Covalent microcontact printing of proteins for cell patterning. Chemistry: a European journal, 12(24), 6290-6297. https://doi.org/10.1002/chem.200501554
Rozkiewicz, D.I. ; Kraan, Yvonne M. ; Werten, Marc W.T. ; de Wolf, Frits A. ; Subramaniam, Vinod ; Ravoo, B.J. ; Reinhoudt, David. / Covalent microcontact printing of proteins for cell patterning. In: Chemistry: a European journal. 2006 ; Vol. 12, No. 24. pp. 6290-6297.
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Rozkiewicz, DI, Kraan, YM, Werten, MWT, de Wolf, FA, Subramaniam, V, Ravoo, BJ & Reinhoudt, D 2006, 'Covalent microcontact printing of proteins for cell patterning' Chemistry: a European journal, vol. 12, no. 24, pp. 6290-6297. https://doi.org/10.1002/chem.200501554

Covalent microcontact printing of proteins for cell patterning. / Rozkiewicz, D.I.; Kraan, Yvonne M.; Werten, Marc W.T.; de Wolf, Frits A.; Subramaniam, Vinod; Ravoo, B.J.; Reinhoudt, David.

In: Chemistry: a European journal, Vol. 12, No. 24, 2006, p. 6290-6297.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Covalent microcontact printing of proteins for cell patterning

AU - Rozkiewicz, D.I.

AU - Kraan, Yvonne M.

AU - Werten, Marc W.T.

AU - de Wolf, Frits A.

AU - Subramaniam, Vinod

AU - Ravoo, B.J.

AU - Reinhoudt, David

PY - 2006

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AB - We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive islands, the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion.

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KW - PROTEINS

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Rozkiewicz DI, Kraan YM, Werten MWT, de Wolf FA, Subramaniam V, Ravoo BJ et al. Covalent microcontact printing of proteins for cell patterning. Chemistry: a European journal. 2006;12(24):6290-6297. https://doi.org/10.1002/chem.200501554