Creating nanopatterns of his-tagged proteins on surfaces by nanoimprint lithography using specific NiNTA-histidine interactions

P.A. Maury, M. Escalante Marun, M. Péter, David Reinhoudt, Vinod Subramaniam, Jurriaan Huskens

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

Directed assembly of the DsRed FT protein is demonstrated on self-assembled monolayers (SAMs) on silicon substrates patterned by nanoimprint lithography. Initially, the DsRed protein is attached using electrostatic interactions on both topographical (polymer) templates with an amino functionalization and on chemically patterned (flat) substrates. In a second experiment, a patterned NiNTA SAM is used in order to attach the DsRed FT protein via supramolecular interactions, taking advantage of the histidine functionalization of the DsRed FT protein. The NTA SAM is formed on silicon oxide using a multistep covalent process. Patterning of the NTA SAM is performed using nanoimprint lithography. The DsRed FT protein is attached on the patterned NTA layer after treating this with a NiII solution. Moreover, the histidine-NiNTA binding may be reversed by removing the Ni using EDTA or by competition using imidazole. The regeneration and reuse of the substrate by subsequently attaching and removing two different histidine-functionalized proteins from the patterned NTA is shown by fluorescence microscopy.
Original languageUndefined
Pages (from-to)1584-1592
Number of pages8
JournalSmall
Volume3
Issue number9
DOIs
Publication statusPublished - 2007

Keywords

  • Self-Assembly
  • METIS-241378
  • IR-72202
  • PROTEINS
  • Nanopatterning
  • Nanoimprint lithography

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