Cross-linking of dermal sheep collagen using a water-soluble carbodiimide

L.H.H. Olde damink, P.J. Dijkstra, M.J.A. van Luyn, P.B. van Wachem, P. Nieuwenhuis, J. Feijen

Research output: Contribution to journalArticleAcademicpeer-review

560 Citations (Scopus)
1053 Downloads (Pure)


A cross-linking method for collagen-based biomaterials was developed using the water-soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide hydrochloride (EDC). Cross-linking using EDC involves the activation of carboxylic acid groups to give O-acylisourea groups, which form cross-links after reaction with free amine groups. Treatment of dermal sheep collagen (DSC) with EDC (E-DSC) resulted in materials with an increased shrinkage temperature (Ts) and a decreased free amine group content, showing that cross-linking occurred. Addition of N-hydroxysuccinimide to the EDC-containing cross-linking solution (E/N-DSC) increased the rate of cross-linking. Cross-linking increased the Ts of non-cross-linked DSC samples from 56 to 73 °C for E-DSC and to 86 °C for E/N-DSC samples, respectively. For both cross-linking methods a linear relation between the decrease in free amine group content and the increase in Ts was observed. The tensile strength and the high strain modulus of E/N-DSC samples decreased upon cross-linking from 18 to 15MPa and from 26 to 16MPa, respectively. The elongation at break of E/N-DSC increased upon cross-linking from 142 to 180%.
Original languageEnglish
Pages (from-to)765-773
Number of pages8
Issue number8
Publication statusPublished - 1996


  • METIS-105371
  • IR-9854
  • Cross-linking
  • Collagen
  • Carbodiimide


Dive into the research topics of 'Cross-linking of dermal sheep collagen using a water-soluble carbodiimide'. Together they form a unique fingerprint.

Cite this