Crosslinked agarose encapsulated sorbents resistant to steam sterilization. Preparation and mechanical properties

Henk W.M. de Koning, Rob A.F.M. Chamuleau, Adriaan Bantjes

    Research output: Contribution to journal › Article › Academic

    4 Citations (Scopus)
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    Abstract

    The application of agarose in hemoperfusion is hampered by the lack of a suitable sterilization method. A technique has been developed for the crosslinking of agarose encapsulated sorbents by the reaction with 1,3-dichloro-2-propanol (DCP) under strong alkaline conditions. A twofold molar excess of DCP with respect to agarose and an equimolar amount of sodium hydroxide at a concentration of 0.3 mol/L with a reaction time of 1-4 h at 50°C are found to be the optimal conditions. The compressive strength of crosslinked beads is increased by a factor of 4. Agarose capsules are found to degrade by the influence of Y radiation, but are resistant to steam sterilization at 134°C during at least 30 min when crosslinked.
    Original languageEnglish
    Pages (from-to)1-13
    JournalJournal of biomedical materials research
    Volume18
    Issue number1
    DOIs
    Publication statusPublished - 1983

    Fingerprint

    Steam
    Propanol
    Sorbents
    Sepharose
    Mechanical properties
    Crosslinking
    Compressive strength
    Sodium
    Radiation
    Sodium Hydroxide
    Capsules
    1,3-dichloro-2-propanol

    Keywords

    • IR-70739

    Cite this

    de Koning, Henk W.M. ; Chamuleau, Rob A.F.M. ; Bantjes, Adriaan. / Crosslinked agarose encapsulated sorbents resistant to steam sterilization. Preparation and mechanical properties. In: Journal of biomedical materials research. 1983 ; Vol. 18, No. 1. pp. 1-13.
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    abstract = "The application of agarose in hemoperfusion is hampered by the lack of a suitable sterilization method. A technique has been developed for the crosslinking of agarose encapsulated sorbents by the reaction with 1,3-dichloro-2-propanol (DCP) under strong alkaline conditions. A twofold molar excess of DCP with respect to agarose and an equimolar amount of sodium hydroxide at a concentration of 0.3 mol/L with a reaction time of 1-4 h at 50°C are found to be the optimal conditions. The compressive strength of crosslinked beads is increased by a factor of 4. Agarose capsules are found to degrade by the influence of Y radiation, but are resistant to steam sterilization at 134°C during at least 30 min when crosslinked.",
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    de Koning, HWM, Chamuleau, RAFM & Bantjes, A 1983, 'Crosslinked agarose encapsulated sorbents resistant to steam sterilization. Preparation and mechanical properties' Journal of biomedical materials research, vol. 18, no. 1, pp. 1-13. https://doi.org/10.1002/jbm.820180103

    Crosslinked agarose encapsulated sorbents resistant to steam sterilization. Preparation and mechanical properties. / de Koning, Henk W.M.; Chamuleau, Rob A.F.M.; Bantjes, Adriaan.

    In: Journal of biomedical materials research, Vol. 18, No. 1, 1983, p. 1-13.

    Research output: Contribution to journal › Article › Academic

    TY - JOUR

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    AU - Chamuleau, Rob A.F.M.

    AU - Bantjes, Adriaan

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    AB - The application of agarose in hemoperfusion is hampered by the lack of a suitable sterilization method. A technique has been developed for the crosslinking of agarose encapsulated sorbents by the reaction with 1,3-dichloro-2-propanol (DCP) under strong alkaline conditions. A twofold molar excess of DCP with respect to agarose and an equimolar amount of sodium hydroxide at a concentration of 0.3 mol/L with a reaction time of 1-4 h at 50°C are found to be the optimal conditions. The compressive strength of crosslinked beads is increased by a factor of 4. Agarose capsules are found to degrade by the influence of Y radiation, but are resistant to steam sterilization at 134°C during at least 30 min when crosslinked.

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    de Koning HWM, Chamuleau RAFM, Bantjes A. Crosslinked agarose encapsulated sorbents resistant to steam sterilization. Preparation and mechanical properties. Journal of biomedical materials research. 1983;18(1):1-13. https://doi.org/10.1002/jbm.820180103

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