TY - JOUR
T1 - Crosslinked agarose encapsulated sorbents resistant to steam sterilization. Preparation and mechanical properties
AU - de Koning, Henk W.M.
AU - Chamuleau, Rob A.F.M.
AU - Bantjes, Adriaan
PY - 1983
Y1 - 1983
N2 - The application of agarose in hemoperfusion is hampered by the lack of a suitable sterilization method. A technique has been developed for the crosslinking of agarose encapsulated sorbents by the reaction with 1,3-dichloro-2-propanol (DCP) under strong alkaline conditions. A twofold molar excess of DCP with respect to agarose and an equimolar amount of sodium hydroxide at a concentration of 0.3 mol/L with a reaction time of 1-4 h at 50°C are found to be the optimal conditions. The compressive strength of crosslinked beads is increased by a factor of 4. Agarose capsules are found to degrade by the influence of Y radiation, but are resistant to steam sterilization at 134°C during at least 30 min when crosslinked.
AB - The application of agarose in hemoperfusion is hampered by the lack of a suitable sterilization method. A technique has been developed for the crosslinking of agarose encapsulated sorbents by the reaction with 1,3-dichloro-2-propanol (DCP) under strong alkaline conditions. A twofold molar excess of DCP with respect to agarose and an equimolar amount of sodium hydroxide at a concentration of 0.3 mol/L with a reaction time of 1-4 h at 50°C are found to be the optimal conditions. The compressive strength of crosslinked beads is increased by a factor of 4. Agarose capsules are found to degrade by the influence of Y radiation, but are resistant to steam sterilization at 134°C during at least 30 min when crosslinked.
KW - IR-70739
U2 - 10.1002/jbm.820180103
DO - 10.1002/jbm.820180103
M3 - Article
VL - 18
SP - 1
EP - 13
JO - Journal of biomedical materials research
JF - Journal of biomedical materials research
SN - 0021-9304
IS - 1
ER -