Crosslinked collagen/chitosan matrix for artificial livers

X.H. Wang; D.P. Li; W.J. Wang; Q.L. Feng; F.Z. Cui; Y.X. Xu; X.H. Song; Mark van der Werf

doi:10.1016/S0142-9612(03)00170-4

Crosslinked collagen/chitosan matrix for artificial livers

X.H. Wang, D.P. Li, W.J. Wang, Q.L. Feng, F.Z. Cui, Y.X. Xu, X.H. Song, Mark van der Werf

Faculty of Engineering Technology

Research output: Contribution to journal › Article › Academic

318 Citations (Scopus)

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Abstract

Matrices composed of collagen and chitosan may create an appropriate environment for the regeneration of livers. In this study, we have prepared, characterized and evaluated a new collagen/chitosan matrix (CCM). The CCM was made by using crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in N-hydroxysuccinimide (NHS) and a 2-morpholinoethane sulfonic acid (MES) buffer system. The chemical characteristics were evaluated by Fourier-transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by tensile tests. The platelet deposition and hepatocyte culture experiments show that CCM has excellent blood and cell compatibility. The results suggest that the CCM is a promising candidate matrix for implantable bioartificial livers.

Original language	Undefined
Pages (from-to)	3213-3220
Journal	Biomaterials
Volume	24
Issue number	19
DOIs	https://doi.org/10.1016/S0142-9612(03)00170-4
Publication status	Published - 2003

Keywords

IR-75417
Liver
Hepatocyte
1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)
Collagen/chitosan matrix (CCM)

Access to Document

10.1016/S0142-9612(03)00170-4

Cite this

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title = "Crosslinked collagen/chitosan matrix for artificial livers",

abstract = "Matrices composed of collagen and chitosan may create an appropriate environment for the regeneration of livers. In this study, we have prepared, characterized and evaluated a new collagen/chitosan matrix (CCM). The CCM was made by using crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in N-hydroxysuccinimide (NHS) and a 2-morpholinoethane sulfonic acid (MES) buffer system. The chemical characteristics were evaluated by Fourier-transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by tensile tests. The platelet deposition and hepatocyte culture experiments show that CCM has excellent blood and cell compatibility. The results suggest that the CCM is a promising candidate matrix for implantable bioartificial livers.",

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volume = "24",

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TY - JOUR

T1 - Crosslinked collagen/chitosan matrix for artificial livers

AU - Wang, X.H.

AU - Li, D.P.

AU - Wang, W.J.

AU - Feng, Q.L.

AU - Cui, F.Z.

AU - Xu, Y.X.

AU - Song, X.H.

AU - van der Werf, Mark

PY - 2003

Y1 - 2003

N2 - Matrices composed of collagen and chitosan may create an appropriate environment for the regeneration of livers. In this study, we have prepared, characterized and evaluated a new collagen/chitosan matrix (CCM). The CCM was made by using crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in N-hydroxysuccinimide (NHS) and a 2-morpholinoethane sulfonic acid (MES) buffer system. The chemical characteristics were evaluated by Fourier-transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by tensile tests. The platelet deposition and hepatocyte culture experiments show that CCM has excellent blood and cell compatibility. The results suggest that the CCM is a promising candidate matrix for implantable bioartificial livers.

AB - Matrices composed of collagen and chitosan may create an appropriate environment for the regeneration of livers. In this study, we have prepared, characterized and evaluated a new collagen/chitosan matrix (CCM). The CCM was made by using crosslinking agent 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) in N-hydroxysuccinimide (NHS) and a 2-morpholinoethane sulfonic acid (MES) buffer system. The chemical characteristics were evaluated by Fourier-transformed infrared (FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The mechanical strength was measured by tensile tests. The platelet deposition and hepatocyte culture experiments show that CCM has excellent blood and cell compatibility. The results suggest that the CCM is a promising candidate matrix for implantable bioartificial livers.

KW - IR-75417

KW - Liver

KW - Hepatocyte

KW - 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)

KW - Collagen/chitosan matrix (CCM)

U2 - 10.1016/S0142-9612(03)00170-4

DO - 10.1016/S0142-9612(03)00170-4

M3 - Article

SN - 0142-9612

VL - 24

SP - 3213

EP - 3220

JO - Biomaterials

JF - Biomaterials

IS - 19

ER -