Crosslinking of dermal sheep collagen using hexamethylene diisocyanate

L.H.H. Olde damink, P.J. Dijkstra, M.J.A. van Luyn, P.B. van Wachem, P. Nieuwenhuis, J. Feijen

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Abstract

The use of hexamethylene diisocyanate (HMDIC) as a crosslinking agent for dermal sheep collagen (DSC) was studied. Because HMDIC is only slightly water soluble, a surfactant was used to obtain a clear and micellar crosslinking solution and to promote the penetration of HMDIC in the DSC matrix. Using optimized conditions treatment of non-crosslinked DSC (N-DSC) with HMDIC (H-DSC) increased the shrinkage temperature (Ts) of N-DSC from 56°C to 74°C for H-DSC. A linear relation between the decrease in free amine group content and the increase in Ts was observed. Crosslinking with HMDIC did not influence the tensile strength of the N-DSC samples but increased the elongation at break from 141% to 163% and decreased the high-strain modulus from 26 MPa to 16 MPa for the H-DSC samples, respectively.
Original languageEnglish
Pages (from-to)429-434
JournalJournal of materials science. Materials in medicine
Volume6
Issue number7
DOIs
Publication statusPublished - 1995

Fingerprint

Collagen
Crosslinking
Sheep
Skin
Tensile Strength
Surface-Active Agents
Amines
1,6-hexamethylene diisocyanate
Elongation
Surface active agents
Tensile strength
Temperature
Water

Keywords

  • METIS-105354
  • IR-85773

Cite this

Olde damink, L.H.H. ; Dijkstra, P.J. ; van Luyn, M.J.A. ; van Wachem, P.B. ; Nieuwenhuis, P. ; Feijen, J. / Crosslinking of dermal sheep collagen using hexamethylene diisocyanate. In: Journal of materials science. Materials in medicine. 1995 ; Vol. 6, No. 7. pp. 429-434.
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title = "Crosslinking of dermal sheep collagen using hexamethylene diisocyanate",
abstract = "The use of hexamethylene diisocyanate (HMDIC) as a crosslinking agent for dermal sheep collagen (DSC) was studied. Because HMDIC is only slightly water soluble, a surfactant was used to obtain a clear and micellar crosslinking solution and to promote the penetration of HMDIC in the DSC matrix. Using optimized conditions treatment of non-crosslinked DSC (N-DSC) with HMDIC (H-DSC) increased the shrinkage temperature (Ts) of N-DSC from 56°C to 74°C for H-DSC. A linear relation between the decrease in free amine group content and the increase in Ts was observed. Crosslinking with HMDIC did not influence the tensile strength of the N-DSC samples but increased the elongation at break from 141{\%} to 163{\%} and decreased the high-strain modulus from 26 MPa to 16 MPa for the H-DSC samples, respectively.",
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year = "1995",
doi = "10.1007/BF00120286",
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Crosslinking of dermal sheep collagen using hexamethylene diisocyanate. / Olde damink, L.H.H.; Dijkstra, P.J.; van Luyn, M.J.A.; van Wachem, P.B.; Nieuwenhuis, P.; Feijen, J.

In: Journal of materials science. Materials in medicine, Vol. 6, No. 7, 1995, p. 429-434.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Crosslinking of dermal sheep collagen using hexamethylene diisocyanate

AU - Olde damink, L.H.H.

AU - Dijkstra, P.J.

AU - van Luyn, M.J.A.

AU - van Wachem, P.B.

AU - Nieuwenhuis, P.

AU - Feijen, J.

PY - 1995

Y1 - 1995

N2 - The use of hexamethylene diisocyanate (HMDIC) as a crosslinking agent for dermal sheep collagen (DSC) was studied. Because HMDIC is only slightly water soluble, a surfactant was used to obtain a clear and micellar crosslinking solution and to promote the penetration of HMDIC in the DSC matrix. Using optimized conditions treatment of non-crosslinked DSC (N-DSC) with HMDIC (H-DSC) increased the shrinkage temperature (Ts) of N-DSC from 56°C to 74°C for H-DSC. A linear relation between the decrease in free amine group content and the increase in Ts was observed. Crosslinking with HMDIC did not influence the tensile strength of the N-DSC samples but increased the elongation at break from 141% to 163% and decreased the high-strain modulus from 26 MPa to 16 MPa for the H-DSC samples, respectively.

AB - The use of hexamethylene diisocyanate (HMDIC) as a crosslinking agent for dermal sheep collagen (DSC) was studied. Because HMDIC is only slightly water soluble, a surfactant was used to obtain a clear and micellar crosslinking solution and to promote the penetration of HMDIC in the DSC matrix. Using optimized conditions treatment of non-crosslinked DSC (N-DSC) with HMDIC (H-DSC) increased the shrinkage temperature (Ts) of N-DSC from 56°C to 74°C for H-DSC. A linear relation between the decrease in free amine group content and the increase in Ts was observed. Crosslinking with HMDIC did not influence the tensile strength of the N-DSC samples but increased the elongation at break from 141% to 163% and decreased the high-strain modulus from 26 MPa to 16 MPa for the H-DSC samples, respectively.

KW - METIS-105354

KW - IR-85773

U2 - 10.1007/BF00120286

DO - 10.1007/BF00120286

M3 - Article

VL - 6

SP - 429

EP - 434

JO - Journal of materials science. Materials in medicine

JF - Journal of materials science. Materials in medicine

SN - 0957-4530

IS - 7

ER -