Currently, efforts are being made to reduce xenograft calcification and to optimize biomechanical properties by applying different crosslinking methods and techniques. Porcine aortic leaflets could be stabilized with a bisepoxy compound, butane-1,4-diol diglycidyl ether (BDDGE), under acidic (pH 4.5) or alkaline conditions (pH > 8.5). Maximum values of the shrinkage temperature (83°C), which are comparable to glutaraldehyde-crosslinked materials, are achieved at pH 9.0 and 10.0 within 48 h with a BDDGE concentration of 4 wt6percnt;. The crosslinking efficacy decreased at higher pH values or at prolonged reaction times due to a higher proportion of one-side or masking reactions. Crosslinking of aortic leaflets at pH 4.5, which consisted in a reaction between the epoxide groups of BDDGE and the carboxylic acid groups of the tissue, afforded crosslinked material with a shrinkage temperature of 76°C after 7 days of reaction.
|Title of host publication||Polymers in medicine|
|Editors||J. dr. Kahovec, I. Meisel, C.S. Kniep, S. Spiegel|
|Place of Publication||Weinheim|
|ISBN (Print)||3-527-30 334-0|
|Publication status||Published - 17 Jul 2001|
|Publisher||International Union of Pure and Applied Chemistry|
Dijkstra, P. J., Zeeman, R., & Feijen, J. (2001). Crosslinking of porcine aortic leaflets with butane-1,4-diol diglycidyl ether. In J. dr. Kahovec, I. Meisel, C. S. Kniep, & S. Spiegel (Eds.), Polymers in medicine (pp. 11-20). Weinheim: Wiley-VCH Verlag. https://doi.org/10.1002/1521-3900(200107)172:1<11::AID-MASY11>3.0.CO;2-E