Crown ether activation of cross-linked subtilisin Carlsberg crystals in organic solvents

Dirk-Jan van Unen, Inna K. Sakodinskaya, Johan F.J. Engbersen, David N. Reinhoudt

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

The activity of cross-linked subtilisin Carlsberg crystals in the catalysis of peptide bond formation can be significantly enhanced by pretreatment of the enzyme crystals with crown ethers. Soaking of the enzyme crystals in a solution of crown ether in acetonitrile followed by evaporation of the solvent results in an up to 13 times enhanced enzymatic activity. The effects of crown ether treatment under various conditions gives support for the hypothesis that removal of bound water molecules from the active site during the drying process is the origin of the observed enzyme activation.
Original languageEnglish
Pages (from-to)3341-3343
JournalJournal of the Chemical Society. Perkin transactions I
Volume1998
Issue number20
DOIs
Publication statusPublished - 1998

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