Cutoff levels of 17-α-hydroxyprogesterone in neonatal screening for congenital adrenal hyperplasia should be based on gestational age rather than on birth weight

Hetty J. van der Kamp, Caren G.M. Groothuis-Oudshoorn, Bert H. Elvers, Maja van Baarle, Barto J. Otten, Jan M. Wit, Paul H. Verkerk

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Abstract

Objective: In newborn screening programs for congenital adrenal hyperplasia, 17-α-hydroxyprogesterone (17OHP) cutoff levels are based on birth weight (BW) or on gestational age (GA). We investigated which approach would result in the greatest specificity and sensitivity.

Study design: For the determination of 17OHP, a neonatal 17OHP assay was used in filter paper blood of 9492 newborns. The relationships between 17OHP and BW and between 17OHP and GA were studied by regression analysis. Reference curves with a specificity of 99.95% were constructed with the method that summarizes the distribution by three smoothed curves representing the skewness (L curve), the median (M curve), and the coefficient of variation (S curve). Median cutoff levels for BW and for GA according to the 99.95% reference curves were calculated.

Results: Regression analysis showed that GA is a better predictor of 17OHP than BW (R 2 was 50.6 vs. 35.8%, respectively). At a specificity of 99.95%, the calculated median 17OHP cutoff level was lower for GA [12.6 μg/liter (38 nmol/liter)] than for BW [17.6 μg/liter (54 nmol/liter)], thus leading to a greater sensitivity.

Conclusion: This study demonstrates that GA is a better predictor of 17OHP in newborns and will result in greater specificity than BW despite the fact that the determination of GA might be less reliable than BW.

Original languageEnglish
Pages (from-to)3904-3907
Number of pages4
JournalJournal of clinical endocrinology and metabolism
Volume90
Issue number7
DOIs
Publication statusPublished - Jul 2005
Externally publishedYes

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17-alpha-Hydroxyprogesterone
Neonatal Screening
Congenital Adrenal Hyperplasia
Birth Weight
Regression analysis
Gestational Age
Screening
Assays
Blood
Regression Analysis
Sensitivity and Specificity

Cite this

@article{0ef5da4f4e0b4450b08f1d3851a52cfa,
title = "Cutoff levels of 17-α-hydroxyprogesterone in neonatal screening for congenital adrenal hyperplasia should be based on gestational age rather than on birth weight",
abstract = "Objective: In newborn screening programs for congenital adrenal hyperplasia, 17-α-hydroxyprogesterone (17OHP) cutoff levels are based on birth weight (BW) or on gestational age (GA). We investigated which approach would result in the greatest specificity and sensitivity.Study design: For the determination of 17OHP, a neonatal 17OHP assay was used in filter paper blood of 9492 newborns. The relationships between 17OHP and BW and between 17OHP and GA were studied by regression analysis. Reference curves with a specificity of 99.95{\%} were constructed with the method that summarizes the distribution by three smoothed curves representing the skewness (L curve), the median (M curve), and the coefficient of variation (S curve). Median cutoff levels for BW and for GA according to the 99.95{\%} reference curves were calculated.Results: Regression analysis showed that GA is a better predictor of 17OHP than BW (R 2 was 50.6 vs. 35.8{\%}, respectively). At a specificity of 99.95{\%}, the calculated median 17OHP cutoff level was lower for GA [12.6 μg/liter (38 nmol/liter)] than for BW [17.6 μg/liter (54 nmol/liter)], thus leading to a greater sensitivity.Conclusion: This study demonstrates that GA is a better predictor of 17OHP in newborns and will result in greater specificity than BW despite the fact that the determination of GA might be less reliable than BW.",
author = "{van der Kamp}, {Hetty J.} and Groothuis-Oudshoorn, {Caren G.M.} and Elvers, {Bert H.} and {van Baarle}, Maja and Otten, {Barto J.} and Wit, {Jan M.} and Verkerk, {Paul H.}",
year = "2005",
month = "7",
doi = "10.1210/jc.2004-2136",
language = "English",
volume = "90",
pages = "3904--3907",
journal = "Journal of clinical endocrinology and metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "7",

}

Cutoff levels of 17-α-hydroxyprogesterone in neonatal screening for congenital adrenal hyperplasia should be based on gestational age rather than on birth weight. / van der Kamp, Hetty J.; Groothuis-Oudshoorn, Caren G.M.; Elvers, Bert H.; van Baarle, Maja; Otten, Barto J.; Wit, Jan M.; Verkerk, Paul H.

In: Journal of clinical endocrinology and metabolism, Vol. 90, No. 7, 07.2005, p. 3904-3907.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cutoff levels of 17-α-hydroxyprogesterone in neonatal screening for congenital adrenal hyperplasia should be based on gestational age rather than on birth weight

AU - van der Kamp, Hetty J.

AU - Groothuis-Oudshoorn, Caren G.M.

AU - Elvers, Bert H.

AU - van Baarle, Maja

AU - Otten, Barto J.

AU - Wit, Jan M.

AU - Verkerk, Paul H.

PY - 2005/7

Y1 - 2005/7

N2 - Objective: In newborn screening programs for congenital adrenal hyperplasia, 17-α-hydroxyprogesterone (17OHP) cutoff levels are based on birth weight (BW) or on gestational age (GA). We investigated which approach would result in the greatest specificity and sensitivity.Study design: For the determination of 17OHP, a neonatal 17OHP assay was used in filter paper blood of 9492 newborns. The relationships between 17OHP and BW and between 17OHP and GA were studied by regression analysis. Reference curves with a specificity of 99.95% were constructed with the method that summarizes the distribution by three smoothed curves representing the skewness (L curve), the median (M curve), and the coefficient of variation (S curve). Median cutoff levels for BW and for GA according to the 99.95% reference curves were calculated.Results: Regression analysis showed that GA is a better predictor of 17OHP than BW (R 2 was 50.6 vs. 35.8%, respectively). At a specificity of 99.95%, the calculated median 17OHP cutoff level was lower for GA [12.6 μg/liter (38 nmol/liter)] than for BW [17.6 μg/liter (54 nmol/liter)], thus leading to a greater sensitivity.Conclusion: This study demonstrates that GA is a better predictor of 17OHP in newborns and will result in greater specificity than BW despite the fact that the determination of GA might be less reliable than BW.

AB - Objective: In newborn screening programs for congenital adrenal hyperplasia, 17-α-hydroxyprogesterone (17OHP) cutoff levels are based on birth weight (BW) or on gestational age (GA). We investigated which approach would result in the greatest specificity and sensitivity.Study design: For the determination of 17OHP, a neonatal 17OHP assay was used in filter paper blood of 9492 newborns. The relationships between 17OHP and BW and between 17OHP and GA were studied by regression analysis. Reference curves with a specificity of 99.95% were constructed with the method that summarizes the distribution by three smoothed curves representing the skewness (L curve), the median (M curve), and the coefficient of variation (S curve). Median cutoff levels for BW and for GA according to the 99.95% reference curves were calculated.Results: Regression analysis showed that GA is a better predictor of 17OHP than BW (R 2 was 50.6 vs. 35.8%, respectively). At a specificity of 99.95%, the calculated median 17OHP cutoff level was lower for GA [12.6 μg/liter (38 nmol/liter)] than for BW [17.6 μg/liter (54 nmol/liter)], thus leading to a greater sensitivity.Conclusion: This study demonstrates that GA is a better predictor of 17OHP in newborns and will result in greater specificity than BW despite the fact that the determination of GA might be less reliable than BW.

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U2 - 10.1210/jc.2004-2136

DO - 10.1210/jc.2004-2136

M3 - Article

VL - 90

SP - 3904

EP - 3907

JO - Journal of clinical endocrinology and metabolism

JF - Journal of clinical endocrinology and metabolism

SN - 0021-972X

IS - 7

ER -