Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications

Brigitte Bruijns*, Andrea Veciana, Roald Tiggelaar, Han Gardeniers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)
9 Downloads (Pure)

Abstract

Microfluidic devices offer important benefits for forensic applications, in particular for fast tests at a crime scene. A large portion of forensic applications require microfluidic chip material to show compatibility with biochemical reactions (such as amplification reactions), and to have high transparency in the visible region and high chemical resistance. Also, preferably, manufacturing should be simple. The characteristic properties of cyclic olefin copolymer (COC) fulfills these requirements and offers new opportunities for the development of new forensic tests. In this work, the versatility of COC as material for lab-on-a-chip (LOC) systems in forensic applications has been explored by realizing two proof-of-principle devices. Chemical resistance and optical transparency were investigated for the development of an on-chip presumptive color test to indicate the presence of an illicit substance through applying absorption spectroscopy. Furthermore, the compatibility of COC with a DNA amplification reaction was verified by performing an on-chip multiple displacement amplification (MDA) reaction.
Original languageEnglish
Article number85
JournalBiosensors
Volume9
Issue number3
DOIs
Publication statusPublished - 4 Jul 2019

Keywords

  • forensic science
  • microfluidic device
  • illicit drug analysis
  • presumptive forensic test
  • UV-VIS spectroscopy
  • cyclic olefin copolymer
  • polymer bonding
  • polymer surface functionalization

Cite this

Bruijns, Brigitte ; Veciana, Andrea ; Tiggelaar, Roald ; Gardeniers, Han. / Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications. In: Biosensors. 2019 ; Vol. 9, No. 3.
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Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications. / Bruijns, Brigitte; Veciana, Andrea; Tiggelaar, Roald; Gardeniers, Han.

In: Biosensors, Vol. 9, No. 3, 85, 04.07.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Cyclic Olefin Copolymer Microfluidic Devices for Forensic Applications

AU - Bruijns, Brigitte

AU - Veciana, Andrea

AU - Tiggelaar, Roald

AU - Gardeniers, Han

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Y1 - 2019/7/4

N2 - Microfluidic devices offer important benefits for forensic applications, in particular for fast tests at a crime scene. A large portion of forensic applications require microfluidic chip material to show compatibility with biochemical reactions (such as amplification reactions), and to have high transparency in the visible region and high chemical resistance. Also, preferably, manufacturing should be simple. The characteristic properties of cyclic olefin copolymer (COC) fulfills these requirements and offers new opportunities for the development of new forensic tests. In this work, the versatility of COC as material for lab-on-a-chip (LOC) systems in forensic applications has been explored by realizing two proof-of-principle devices. Chemical resistance and optical transparency were investigated for the development of an on-chip presumptive color test to indicate the presence of an illicit substance through applying absorption spectroscopy. Furthermore, the compatibility of COC with a DNA amplification reaction was verified by performing an on-chip multiple displacement amplification (MDA) reaction.

AB - Microfluidic devices offer important benefits for forensic applications, in particular for fast tests at a crime scene. A large portion of forensic applications require microfluidic chip material to show compatibility with biochemical reactions (such as amplification reactions), and to have high transparency in the visible region and high chemical resistance. Also, preferably, manufacturing should be simple. The characteristic properties of cyclic olefin copolymer (COC) fulfills these requirements and offers new opportunities for the development of new forensic tests. In this work, the versatility of COC as material for lab-on-a-chip (LOC) systems in forensic applications has been explored by realizing two proof-of-principle devices. Chemical resistance and optical transparency were investigated for the development of an on-chip presumptive color test to indicate the presence of an illicit substance through applying absorption spectroscopy. Furthermore, the compatibility of COC with a DNA amplification reaction was verified by performing an on-chip multiple displacement amplification (MDA) reaction.

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KW - illicit drug analysis

KW - presumptive forensic test

KW - UV-VIS spectroscopy

KW - cyclic olefin copolymer

KW - polymer bonding

KW - polymer surface functionalization

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