Cytocompatible carbon nanotube reinforced polyethylene glycol composite hydrogels for tissue engineering

Laurien Van den Broeck, Susanna Piluso, Al Halifa Soultan, Michael De Volder, Jennifer Patterson (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

Hydrogels are attractive materials for stimulating 3D cell growth and tissue regeneration, and they provide mechanical support and physical cues to guide cell behavior. Herein, we developed a robust methodology to increase the stiffness of polyethylene glycol (PEG) hydrogels by successfully incorporating carbon nanotubes (CNTs) within the polymer matrix. Interestingly, hydrogels containing pristine CNTs showed a higher stiffness (1915 ± 102 Pa) than both hydrogels without CNTs (1197 ± 125 Pa) and hydrogels incorporating PEG-grafted CNTs (867 ± 103 Pa) (p < 0.005). The swelling ratio was lower for hydrogels with pristine CNTs (45.4 ± 3.5) and hydrogels without CNTs (46.7 ± 5.1) compared to the hydrogels with PEG-grafted CNTs (62.8 ± 2.6). To confirm that the CNT-reinforced hydrogels were cytocompatible, the viability, proliferation, and morphology of encapsulated L929 fibroblasts was investigated. All hydrogel formulations supported cell proliferation, and the addition of pristine CNTs increased initial cell viability (83.3 ± 10.7%) compared to both pure PEG hydrogels (51.9 ± 8.3%) and hydrogels with PEG-CNTs (63.1 ± 10.9%) (p < 0.005). Altogether, these results demonstrate that incorporation of CNTs could effectively reinforce PEG hydrogels and that the resulting cytocompatible nanocomposites are promising scaffolds for tissue engineering.

Original languageEnglish
Pages (from-to)1133-1144
Number of pages12
JournalMaterials Science and Engineering C
Volume98
DOIs
Publication statusPublished - 1 May 2019
Externally publishedYes

Fingerprint

Carbon Nanotubes
Hydrogels
tissue engineering
Tissue engineering
Polyethylene glycols
glycols
polyethylenes
Carbon nanotubes
carbon nanotubes
composite materials
Composite materials
viability
stiffness
Stiffness
Tissue regeneration
cues
fibroblasts
Hydrogel
Cell proliferation
regeneration

Keywords

  • Cytocompatibility
  • Hydrogel swelling
  • Mechanical properties
  • Multi-walled carbon nanotubes
  • PEGylation

Cite this

Van den Broeck, Laurien ; Piluso, Susanna ; Soultan, Al Halifa ; De Volder, Michael ; Patterson, Jennifer. / Cytocompatible carbon nanotube reinforced polyethylene glycol composite hydrogels for tissue engineering. In: Materials Science and Engineering C. 2019 ; Vol. 98. pp. 1133-1144.
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Cytocompatible carbon nanotube reinforced polyethylene glycol composite hydrogels for tissue engineering. / Van den Broeck, Laurien; Piluso, Susanna; Soultan, Al Halifa; De Volder, Michael; Patterson, Jennifer (Corresponding Author).

In: Materials Science and Engineering C, Vol. 98, 01.05.2019, p. 1133-1144.

Research output: Contribution to journalArticleAcademicpeer-review

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