Abstract
Hydrogels are attractive materials for stimulating 3D cell growth and tissue regeneration, and they provide mechanical support and physical cues to guide cell behavior. Herein, we developed a robust methodology to increase the stiffness of polyethylene glycol (PEG) hydrogels by successfully incorporating carbon nanotubes (CNTs) within the polymer matrix. Interestingly, hydrogels containing pristine CNTs showed a higher stiffness (1915 ± 102 Pa) than both hydrogels without CNTs (1197 ± 125 Pa) and hydrogels incorporating PEG-grafted CNTs (867 ± 103 Pa) (p < 0.005). The swelling ratio was lower for hydrogels with pristine CNTs (45.4 ± 3.5) and hydrogels without CNTs (46.7 ± 5.1) compared to the hydrogels with PEG-grafted CNTs (62.8 ± 2.6). To confirm that the CNT-reinforced hydrogels were cytocompatible, the viability, proliferation, and morphology of encapsulated L929 fibroblasts was investigated. All hydrogel formulations supported cell proliferation, and the addition of pristine CNTs increased initial cell viability (83.3 ± 10.7%) compared to both pure PEG hydrogels (51.9 ± 8.3%) and hydrogels with PEG-CNTs (63.1 ± 10.9%) (p < 0.005). Altogether, these results demonstrate that incorporation of CNTs could effectively reinforce PEG hydrogels and that the resulting cytocompatible nanocomposites are promising scaffolds for tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 1133-1144 |
| Number of pages | 12 |
| Journal | Materials Science and Engineering C |
| Volume | 98 |
| DOIs | |
| Publication status | Published - 1 May 2019 |
| Externally published | Yes |
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Keywords
- Cytocompatibility
- Hydrogel swelling
- Mechanical properties
- Multi-walled carbon nanotubes
- PEGylation
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Cytocompatible carbon nanotube reinforced polyethylene glycol composite hydrogels for tissue engineering. / Van den Broeck, Laurien; Piluso, Susanna; Soultan, Al Halifa; De Volder, Michael; Patterson, Jennifer (Corresponding Author).
In: Materials Science and Engineering C, Vol. 98, 01.05.2019, p. 1133-1144.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Cytocompatible carbon nanotube reinforced polyethylene glycol composite hydrogels for tissue engineering
AU - Van den Broeck, Laurien
AU - Piluso, Susanna
AU - Soultan, Al Halifa
AU - De Volder, Michael
AU - Patterson, Jennifer
PY - 2019/5/1
Y1 - 2019/5/1
N2 - Hydrogels are attractive materials for stimulating 3D cell growth and tissue regeneration, and they provide mechanical support and physical cues to guide cell behavior. Herein, we developed a robust methodology to increase the stiffness of polyethylene glycol (PEG) hydrogels by successfully incorporating carbon nanotubes (CNTs) within the polymer matrix. Interestingly, hydrogels containing pristine CNTs showed a higher stiffness (1915 ± 102 Pa) than both hydrogels without CNTs (1197 ± 125 Pa) and hydrogels incorporating PEG-grafted CNTs (867 ± 103 Pa) (p < 0.005). The swelling ratio was lower for hydrogels with pristine CNTs (45.4 ± 3.5) and hydrogels without CNTs (46.7 ± 5.1) compared to the hydrogels with PEG-grafted CNTs (62.8 ± 2.6). To confirm that the CNT-reinforced hydrogels were cytocompatible, the viability, proliferation, and morphology of encapsulated L929 fibroblasts was investigated. All hydrogel formulations supported cell proliferation, and the addition of pristine CNTs increased initial cell viability (83.3 ± 10.7%) compared to both pure PEG hydrogels (51.9 ± 8.3%) and hydrogels with PEG-CNTs (63.1 ± 10.9%) (p < 0.005). Altogether, these results demonstrate that incorporation of CNTs could effectively reinforce PEG hydrogels and that the resulting cytocompatible nanocomposites are promising scaffolds for tissue engineering.
AB - Hydrogels are attractive materials for stimulating 3D cell growth and tissue regeneration, and they provide mechanical support and physical cues to guide cell behavior. Herein, we developed a robust methodology to increase the stiffness of polyethylene glycol (PEG) hydrogels by successfully incorporating carbon nanotubes (CNTs) within the polymer matrix. Interestingly, hydrogels containing pristine CNTs showed a higher stiffness (1915 ± 102 Pa) than both hydrogels without CNTs (1197 ± 125 Pa) and hydrogels incorporating PEG-grafted CNTs (867 ± 103 Pa) (p < 0.005). The swelling ratio was lower for hydrogels with pristine CNTs (45.4 ± 3.5) and hydrogels without CNTs (46.7 ± 5.1) compared to the hydrogels with PEG-grafted CNTs (62.8 ± 2.6). To confirm that the CNT-reinforced hydrogels were cytocompatible, the viability, proliferation, and morphology of encapsulated L929 fibroblasts was investigated. All hydrogel formulations supported cell proliferation, and the addition of pristine CNTs increased initial cell viability (83.3 ± 10.7%) compared to both pure PEG hydrogels (51.9 ± 8.3%) and hydrogels with PEG-CNTs (63.1 ± 10.9%) (p < 0.005). Altogether, these results demonstrate that incorporation of CNTs could effectively reinforce PEG hydrogels and that the resulting cytocompatible nanocomposites are promising scaffolds for tissue engineering.
KW - Cytocompatibility
KW - Hydrogel swelling
KW - Mechanical properties
KW - Multi-walled carbon nanotubes
KW - PEGylation
UR - http://www.scopus.com/inward/record.url?scp=85060223728&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2019.01.020
DO - 10.1016/j.msec.2019.01.020
M3 - Article
VL - 98
SP - 1133
EP - 1144
JO - Materials science & engineering C
JF - Materials science & engineering C
SN - 0928-4931
ER -