Defining the baseline transcriptional fingerprint of rabbit hamstring autograft

Mario Hevesi, Christopher R. Paradise, Carlo A. Paggi, Catalina Galeano-Garces, Amel Dudakovic, Marcel Karperien, Sanjeev Kakar, Timothy E. Hewett, Aaron J. Krych, Andre J. van Wijnen (Corresponding Author), Daniel B.F. Saris (Corresponding Author)

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Anterior cruciate ligament (ACL) injuries are common and of high relevance given their significant effects on patient function, quality of life, and posttraumatic arthritis. To date, investigators have reported on the expression of genes classically associated with tendon and ligament reconstruction, including decorin (DCN) and collagen type 1 (COL1A1 and COL1A2). However, the transcriptional fingerprint for hamstring tendons, one of the most common autografts used for ACLR, remains to be determined. The purpose of this study was to characterize the baseline transcriptional state of semitendinosus autografts in a rabbit model for ACLR and to employ such characterization to guide scientifically-driven target gene selection for future analyses. Next generation RNA sequencing was performed on whole semitendinosus autografts from four New Zealand White rabbits (mean age: 193 ± 0 days, mean weight: 2.78 kg ± 0.15 kg) and subsequently analyzed using gene enrichment and protein-protein interaction network analysis. Decorin, Secreted Protein Acidic and Cysteine Rich (SPARC), Collagen type 1, and Proline and Arginine Rich End Leucine Rich Repeat Protein (PRELP) and were determined to be the highest expressed genes with tendon-associated ontology. These results strengthen the association between genes such as DCN, COL1A1, and COL1A2 and tendon tissues as well as provide the novel addition of further high-expression, tendon characteristic genes such as SPARC and PRELP to provide guidance as to which molecules serve as high-signal candidates for future ACL research. In addition, this paper provides open-access to the expression fingerprint of hamstring autograft for ACLR in New Zealand White rabbits, thus providing a readily-accessible collaborative reference, in alignment with ethical animal research principles.

Original languageEnglish
Article number100363
JournalGene Reports
Volume15
DOIs
Publication statusPublished - 1 Jun 2019

Fingerprint

Autografts
Dermatoglyphics
Decorin
Tendons
Rabbits
Collagen Type I
Genes
Cysteine
RNA Sequence Analysis
Protein Interaction Maps
Proteins
Anterior Cruciate Ligament
Ligaments
Proline
Arthritis
Arginine
Quality of Life
Research Personnel
Gene Expression
Weights and Measures

Keywords

  • ACL
  • PRELP
  • Rabbit
  • RNA sequencing
  • SPARC
  • Transcriptional fingerprint

Cite this

Hevesi, Mario ; Paradise, Christopher R. ; Paggi, Carlo A. ; Galeano-Garces, Catalina ; Dudakovic, Amel ; Karperien, Marcel ; Kakar, Sanjeev ; Hewett, Timothy E. ; Krych, Aaron J. ; van Wijnen, Andre J. ; Saris, Daniel B.F. / Defining the baseline transcriptional fingerprint of rabbit hamstring autograft. In: Gene Reports. 2019 ; Vol. 15.
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abstract = "Anterior cruciate ligament (ACL) injuries are common and of high relevance given their significant effects on patient function, quality of life, and posttraumatic arthritis. To date, investigators have reported on the expression of genes classically associated with tendon and ligament reconstruction, including decorin (DCN) and collagen type 1 (COL1A1 and COL1A2). However, the transcriptional fingerprint for hamstring tendons, one of the most common autografts used for ACLR, remains to be determined. The purpose of this study was to characterize the baseline transcriptional state of semitendinosus autografts in a rabbit model for ACLR and to employ such characterization to guide scientifically-driven target gene selection for future analyses. Next generation RNA sequencing was performed on whole semitendinosus autografts from four New Zealand White rabbits (mean age: 193 ± 0 days, mean weight: 2.78 kg ± 0.15 kg) and subsequently analyzed using gene enrichment and protein-protein interaction network analysis. Decorin, Secreted Protein Acidic and Cysteine Rich (SPARC), Collagen type 1, and Proline and Arginine Rich End Leucine Rich Repeat Protein (PRELP) and were determined to be the highest expressed genes with tendon-associated ontology. These results strengthen the association between genes such as DCN, COL1A1, and COL1A2 and tendon tissues as well as provide the novel addition of further high-expression, tendon characteristic genes such as SPARC and PRELP to provide guidance as to which molecules serve as high-signal candidates for future ACL research. In addition, this paper provides open-access to the expression fingerprint of hamstring autograft for ACLR in New Zealand White rabbits, thus providing a readily-accessible collaborative reference, in alignment with ethical animal research principles.",
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Hevesi, M, Paradise, CR, Paggi, CA, Galeano-Garces, C, Dudakovic, A, Karperien, M, Kakar, S, Hewett, TE, Krych, AJ, van Wijnen, AJ & Saris, DBF 2019, 'Defining the baseline transcriptional fingerprint of rabbit hamstring autograft' Gene Reports, vol. 15, 100363. https://doi.org/10.1016/j.genrep.2019.100363

Defining the baseline transcriptional fingerprint of rabbit hamstring autograft. / Hevesi, Mario; Paradise, Christopher R.; Paggi, Carlo A.; Galeano-Garces, Catalina; Dudakovic, Amel; Karperien, Marcel; Kakar, Sanjeev; Hewett, Timothy E.; Krych, Aaron J.; van Wijnen, Andre J. (Corresponding Author); Saris, Daniel B.F. (Corresponding Author).

In: Gene Reports, Vol. 15, 100363, 01.06.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Defining the baseline transcriptional fingerprint of rabbit hamstring autograft

AU - Hevesi, Mario

AU - Paradise, Christopher R.

AU - Paggi, Carlo A.

AU - Galeano-Garces, Catalina

AU - Dudakovic, Amel

AU - Karperien, Marcel

AU - Kakar, Sanjeev

AU - Hewett, Timothy E.

AU - Krych, Aaron J.

AU - van Wijnen, Andre J.

AU - Saris, Daniel B.F.

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