Delivery of MicroRNAs by plant virus-based nanoparticles to functionally alter the osteogenic differentiation of human mesenchymal stem cells

Fei Xue, Jeroen J.L.M. Cornelissen, Quan Yuan*, Shuqin Cao*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)
100 Downloads (Pure)

Abstract

MicroRNA-26a (miR-26a) has been verified to promote osteogenic differentiation of mesenchymal stem cells in recent years. The main obstacles to its application in bone regeneration are instability in the physiological environment and low efficiency of cellular membrane penetration. To overcome these problems, we constructed a novel plant virus gene delivery system based on Cowpea chlorotic mottle virus (CCMV). By encapsulating miR-26a with purified capsid protein (CP) dimers derived from CCMV, CP-miR-26a (CP26a) virus-like particles (VLPs) were obtained. CP26a retained a structure similar to the native CCMV and protected miR-26a from digestion with its exterior CP. Moreover, CP26a featured similar cellular uptake efficiency, osteogenesis promotion ability, and better biocompatibility compared with Lipofectamine2000-miR-26a (lipo26a), which indicated a promising prospect for CCMV as a novel gene delivery system.

Original languageEnglish
Article number107448
JournalChinese Chemical Letters
Volume34
Issue number1
Early online date22 Apr 2022
DOIs
Publication statusPublished - Jan 2023

Keywords

  • Cowpea chlorotic mottle virus
  • Gene delivery
  • MicroRNAs
  • Osteogenesis
  • VLPs
  • 2024 OA procedure

Fingerprint

Dive into the research topics of 'Delivery of MicroRNAs by plant virus-based nanoparticles to functionally alter the osteogenic differentiation of human mesenchymal stem cells'. Together they form a unique fingerprint.

Cite this