Design and implementation of injector/distributor structures for microfabricated non-porous pillar columns for capillary electrochromatography

S. Sukas, G. Desmet, Johannes G.E. Gardeniers

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

A previously proposed foil definition is applied in the design of injector/distributor structures for solid microfabricated column structures for capillary electrochromatography. In addition to a typical bifurcated distributor, an optimized design alternative with two different configurations is experimentally evaluated. Optimized designs yielded a flat profile for the injected sample with a maximum of 3% variation from the mean width, while it went up to 18% for the typical bifurcated distributor. The implemented electrokinetic injection approach enabled controlling the volume of the injected sample accurately without sacrificing the compactness of the device design. The width of the injected sample was directly proportional to the injection time, namely 165 and 218 μm base widths were obtained for 0.6 and 0.8 s of feeding, respectively. Reducing the external porosity of the distributor by 85% compared to the typical design, optimized distributors caused a decrease in the mean flow velocity of up to 70%. However, having a flat initial plug shape enabled the separation of a mixture of Coumarin 440, 460, 480 and 540 at 1 mm downstream of the injection point in 80 s, while it was even not possible to detect the C440 signal for a typical bifurcated design.
Original languageEnglish
Pages (from-to)80-87
Number of pages8
JournalJournal of chromatography A
Volume1289
DOIs
Publication statusPublished - 2013

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Capillary Electrochromatography
Injections
Equipment Design
Porosity
Flow velocity
Metal foil

Keywords

  • METIS-295630
  • IR-85414

Cite this

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title = "Design and implementation of injector/distributor structures for microfabricated non-porous pillar columns for capillary electrochromatography",
abstract = "A previously proposed foil definition is applied in the design of injector/distributor structures for solid microfabricated column structures for capillary electrochromatography. In addition to a typical bifurcated distributor, an optimized design alternative with two different configurations is experimentally evaluated. Optimized designs yielded a flat profile for the injected sample with a maximum of 3{\%} variation from the mean width, while it went up to 18{\%} for the typical bifurcated distributor. The implemented electrokinetic injection approach enabled controlling the volume of the injected sample accurately without sacrificing the compactness of the device design. The width of the injected sample was directly proportional to the injection time, namely 165 and 218 μm base widths were obtained for 0.6 and 0.8 s of feeding, respectively. Reducing the external porosity of the distributor by 85{\%} compared to the typical design, optimized distributors caused a decrease in the mean flow velocity of up to 70{\%}. However, having a flat initial plug shape enabled the separation of a mixture of Coumarin 440, 460, 480 and 540 at 1 mm downstream of the injection point in 80 s, while it was even not possible to detect the C440 signal for a typical bifurcated design.",
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pages = "80--87",
journal = "Journal of chromatography A",
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Design and implementation of injector/distributor structures for microfabricated non-porous pillar columns for capillary electrochromatography. / Sukas, S.; Desmet, G.; Gardeniers, Johannes G.E.

In: Journal of chromatography A, Vol. 1289, 2013, p. 80-87.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Design and implementation of injector/distributor structures for microfabricated non-porous pillar columns for capillary electrochromatography

AU - Sukas, S.

AU - Desmet, G.

AU - Gardeniers, Johannes G.E.

PY - 2013

Y1 - 2013

N2 - A previously proposed foil definition is applied in the design of injector/distributor structures for solid microfabricated column structures for capillary electrochromatography. In addition to a typical bifurcated distributor, an optimized design alternative with two different configurations is experimentally evaluated. Optimized designs yielded a flat profile for the injected sample with a maximum of 3% variation from the mean width, while it went up to 18% for the typical bifurcated distributor. The implemented electrokinetic injection approach enabled controlling the volume of the injected sample accurately without sacrificing the compactness of the device design. The width of the injected sample was directly proportional to the injection time, namely 165 and 218 μm base widths were obtained for 0.6 and 0.8 s of feeding, respectively. Reducing the external porosity of the distributor by 85% compared to the typical design, optimized distributors caused a decrease in the mean flow velocity of up to 70%. However, having a flat initial plug shape enabled the separation of a mixture of Coumarin 440, 460, 480 and 540 at 1 mm downstream of the injection point in 80 s, while it was even not possible to detect the C440 signal for a typical bifurcated design.

AB - A previously proposed foil definition is applied in the design of injector/distributor structures for solid microfabricated column structures for capillary electrochromatography. In addition to a typical bifurcated distributor, an optimized design alternative with two different configurations is experimentally evaluated. Optimized designs yielded a flat profile for the injected sample with a maximum of 3% variation from the mean width, while it went up to 18% for the typical bifurcated distributor. The implemented electrokinetic injection approach enabled controlling the volume of the injected sample accurately without sacrificing the compactness of the device design. The width of the injected sample was directly proportional to the injection time, namely 165 and 218 μm base widths were obtained for 0.6 and 0.8 s of feeding, respectively. Reducing the external porosity of the distributor by 85% compared to the typical design, optimized distributors caused a decrease in the mean flow velocity of up to 70%. However, having a flat initial plug shape enabled the separation of a mixture of Coumarin 440, 460, 480 and 540 at 1 mm downstream of the injection point in 80 s, while it was even not possible to detect the C440 signal for a typical bifurcated design.

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