Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells

Aliaksei S. Vasilevich, Frederic Mourcin, Anouk Mentink, Frits Hulshof, Nick Beijer, Yiping Zhao, Marloes Levers, Bernke Papenburg, Shantanu Singh, Anne E. Carpenter, Dimitrios Stamatialis, Clemens van Blitterswijk, Karin Tarte, Jan de Boer (Corresponding Author)

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Abstract

Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.
Original languageEnglish
Article number87
Number of pages14
JournalFrontiers in bioengineering and biotechnology
Volume6
DOIs
Publication statusPublished - 28 Jun 2018

Keywords

  • mechanobiology
  • surface topography
  • fibroblastic reticular cells
  • lymph node
  • ICAM-1

Cite this

Vasilevich, Aliaksei S. ; Mourcin, Frederic ; Mentink, Anouk ; Hulshof, Frits ; Beijer, Nick ; Zhao, Yiping ; Levers, Marloes ; Papenburg, Bernke ; Singh, Shantanu ; Carpenter, Anne E. ; Stamatialis, Dimitrios ; van Blitterswijk, Clemens ; Tarte, Karin ; de Boer, Jan. / Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells. In: Frontiers in bioengineering and biotechnology. 2018 ; Vol. 6.
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abstract = "Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.",
keywords = "mechanobiology, surface topography, fibroblastic reticular cells, lymph node, ICAM-1",
author = "Vasilevich, {Aliaksei S.} and Frederic Mourcin and Anouk Mentink and Frits Hulshof and Nick Beijer and Yiping Zhao and Marloes Levers and Bernke Papenburg and Shantanu Singh and Carpenter, {Anne E.} and Dimitrios Stamatialis and {van Blitterswijk}, Clemens and Karin Tarte and {de Boer}, Jan",
year = "2018",
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Vasilevich, AS, Mourcin, F, Mentink, A, Hulshof, F, Beijer, N, Zhao, Y, Levers, M, Papenburg, B, Singh, S, Carpenter, AE, Stamatialis, D, van Blitterswijk, C, Tarte, K & de Boer, J 2018, 'Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells' Frontiers in bioengineering and biotechnology, vol. 6, 87. https://doi.org/10.3389/fbioe.2018.00087

Designed Surface Topographies Control ICAM-1 Expression in Tonsil-Derived Human Stromal Cells. / Vasilevich, Aliaksei S.; Mourcin, Frederic; Mentink, Anouk; Hulshof, Frits; Beijer, Nick; Zhao, Yiping; Levers, Marloes; Papenburg, Bernke; Singh, Shantanu; Carpenter, Anne E.; Stamatialis, Dimitrios; van Blitterswijk, Clemens; Tarte, Karin; de Boer, Jan (Corresponding Author).

In: Frontiers in bioengineering and biotechnology, Vol. 6, 87, 28.06.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Vasilevich, Aliaksei S.

AU - Mourcin, Frederic

AU - Mentink, Anouk

AU - Hulshof, Frits

AU - Beijer, Nick

AU - Zhao, Yiping

AU - Levers, Marloes

AU - Papenburg, Bernke

AU - Singh, Shantanu

AU - Carpenter, Anne E.

AU - Stamatialis, Dimitrios

AU - van Blitterswijk, Clemens

AU - Tarte, Karin

AU - de Boer, Jan

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AB - Fibroblastic reticular cells (FRCs), the T-cell zone stromal cell subtype in the lymph nodes, create a scaffold for adhesion and migration of immune cells, thus allowing them to communicate. Although known to be important for the initiation of immune responses, studies about FRCs and their interactions have been impeded because FRCs are limited in availability and lose their function upon culture expansion. To circumvent these limitations, stromal cell precursors can be mechanotranduced to form mature FRCs. Here, we used a library of designed surface topographies to trigger FRC differentiation from tonsil-derived stromal cells (TSCs). Undifferentiated TSCs were seeded on a TopoChip containing 2176 different topographies in culture medium without differentiation factors, then monitored cell morphology and the levels of ICAM-1, a marker of FRC differentiation. We identified 112 and 72 surfaces that upregulated and downregulated, respectively, ICAM-1 expression. By monitoring cell morphology, and expression of the FRC differentiation marker ICAM-1 via image analysis and machine learning, we discovered correlations between ICAM-1 expression, cell shape and design of surface topographies and confirmed our findings by using flow cytometry. Our findings confirmed that TSCs are mechano-responsive cells and identified particular topographies that can be used to improve FRC differentiation protocols.

KW - mechanobiology

KW - surface topography

KW - fibroblastic reticular cells

KW - lymph node

KW - ICAM-1

U2 - 10.3389/fbioe.2018.00087

DO - 10.3389/fbioe.2018.00087

M3 - Article

VL - 6

JO - Frontiers in bioengineering and biotechnology

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SN - 2296-4185

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