Abstract
The interaction of lithotripter-generated shock waves with adherent cells is investigated using high-speed optical techniques. We show that shock waves permeabilize adherent cells in vitro through the action of cavitation bubbles. The bubbles are formed in the trailing tensile pulse of a lithotripter-generated shock wave where the pressure drops below the vapor pressure. Upon collapse of cavitation bubbles, a strong flow field is generated which accounts for two effects: first, detachment of cells from the substrate; and second, the temporary opening of cell membranes followed by molecular uptake, a process called sonoporation. Comparison of observed cell detachment with results from a theoretical model considering peeling cell detachment by a wall jet-induced shear stress shows reasonable agreement.
Original language | Undefined |
---|---|
Pages (from-to) | 131-138 |
Number of pages | 8 |
Journal | Biochimica et biophysica acta. General subjects |
Volume | 1624 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - 2003 |
Keywords
- METIS-216922
- Shock wave
- IR-75028
- Cell detachment
- Cavitation
- Sonoporation
- Drug delivery