Detection of charged proteins by means of impedance measurements

J. Kruise; J.G. Rispens; P. Bergveld; F.J.B. Kremer; D.A.J. Starmans; J.R. Haak; J. Feijen; D.N. Reinhoudt

doi:10.1016/0925-4005(92)80039-Z

Detection of charged proteins by means of impedance measurements

J. Kruise, J.G. Rispens, P. Bergveld, F.J.B. Kremer, D.A.J. Starmans, J.R. Haak, J. Feijen, D.N. Reinhoudt

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Abstract

The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory.

Original language	English
Pages (from-to)	101-105
Number of pages	6
Journal	Sensors and Actuators B: Chemical
Volume	6
Issue number	1-3
DOIs	https://doi.org/10.1016/0925-4005(92)80039-Z
Publication status	Published - 1992

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title = "Detection of charged proteins by means of impedance measurements",

abstract = "The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory.",

author = "J. Kruise and J.G. Rispens and P. Bergveld and F.J.B. Kremer and D.A.J. Starmans and J.R. Haak and J. Feijen and D.N. Reinhoudt",

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T1 - Detection of charged proteins by means of impedance measurements

AU - Kruise, J.

AU - Rispens, J.G.

AU - Bergveld, P.

AU - Kremer, F.J.B.

AU - Starmans, D.A.J.

AU - Haak, J.R.

AU - Feijen, J.

AU - Reinhoudt, D.N.

PY - 1992

Y1 - 1992

N2 - The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory.

AB - The electrical resistance of a membrane containing charged proteins is a function of both concentration and mobility of the electrolyte ions present in the membrane, and thus it depends on the Donnan effect. We were able to determine the resistance of such a membrane deposited on an ISFET, by means of a simple a.c. impedance measurement. As an example a membrane containing cross-linked lysozyme was used. We found the membrane resistance to be inversely proportional to the fixed charge density, in good agreement with the theoretical description based on the Donnan theory.

U2 - 10.1016/0925-4005(92)80039-Z

DO - 10.1016/0925-4005(92)80039-Z

M3 - Article

SN - 0925-4005

VL - 6

SP - 101

EP - 105

JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

IS - 1-3

ER -

Detection of charged proteins by means of impedance measurements

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