Fluorescence in situ hybridization signals on human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments on a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are well resolved in the near- field fluorescence images, and the exact location of the probes can be correlated to the topography as it is afforded by the shear-force feedback.
|Number of pages||5|
|Publication status||Published - 1996|
|Event||3rd International Conference on Near-Field Optics, NFO-3 1995 - Brno, Czech Republic|
Duration: 9 May 1995 → 11 May 1995
Conference number: 3