Detection of fluorescence in situ hybridization on human metaphase chromosomes by near-field scanning optical microscopy

M.H.P. Moers, A.G.T. Ruiter, A. Jalocha, N.F. van Hulst

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Abstract

Fluorescence in situ hybridization signals o­n human metaphase chromosomes are detected by a near-field scanning optical microscope. This makes it possible to localize and identify several fluorescently labeled genomic DNA fragments o­n a single chromosome with a resolution superior to traditional fluorescence microscopy. Several nucleic acid probes have been used. The hybridization signals are well resolved in the near- field fluorescence images, and the exact location of the probes can be correlated to the topography as it is afforded by the shear-force feedback.
Original languageEnglish
Pages (from-to)279-283
Number of pages5
JournalUltramicroscopy
Volume61
Issue number1-4
DOIs
Publication statusPublished - 1996
Event3rd International Conference on Near-Field Optics, NFO-3 1995 - Brno, Czech Republic
Duration: 9 May 199511 May 1995
Conference number: 3

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